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• W2044642005 abstract "Abstract H+ extrusion and the plasma membrane H+-ATPase activity were analyzed using barley roots exposed to ammonium. H+ extrusion from roots was enhanced 4 h after the addition of 5 mol m-3 ammonium to the medium. However, the stimulation of the plasma membrane H+-ATPase activity occurred at least 8 h after the addition of ammonium. Furthermore, this stimulation was found to be caused by the increase in the content of H+-ATPase proteins based on the results of immunoassay. These results indicate that H+ was actively extruded to the medium through the increase in the amount of plasma membrane H+-ATPase proteins, when barley roots were exposed to ammonium for more than 8 h. Thus, it is assumed that in the long term exposure to ammonium nitrogen, ammonium-induced stimulation of the H+-ATPase activity may be important to maintain intracellular pH in root cells under the acidic conditions associated with the uptake of ammonium." @default.
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• W2044642005 date "1995-03-01" @default.
• W2044642005 modified "2023-09-27" @default.
• W2044642005 title "Stimulation of H⁺extrusion and plasma membrane H⁺-ATPase activity of barley roots by ammonium-treatment" @default.
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• W2044642005 doi "https://doi.org/10.1080/00380768.1995.10419566" @default.
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