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- W2044642956 abstract "The equilibrium constant for the equilibrium between the linear and the cyclic monomer forms of λb2b5c DNA has been measured as a function of temperature in 0·10 M -NaCl, 0·01 M -Na 3 EDTA (pH 7·3) and in 2·0 M -NaCl, 0·01 M -Na 3 EDTA (pH 7·3). Equilibrium was approached from both sides; band sedimentation was used to measure the amounts of the two forms. In 0·13 M -Na + , Δ H = −91 (± 10) kcal. mole −1 , Δ S = −280 (± 30) eu; in 2 M -Na + , Δ H = −85 (± 10) and Δ S = −250 (± 30). The midpoints of the transitions occur at 50·6 and 63·6°C in the two media. From these data, the number of base pairs involved in cyclization is estimated as 10 ± 1. Rate constants for the first-order relaxation of the system to equilibrium have been measured. These results indicate that the rate of cyclization is not controlled by the rate of diffusion of the two ends toward each other but rather by the rate of hydrogen-bond formation. The activation energy for the cyclization process is about 23 kcal. mole −1 . The equations, log 10 k (min −1 ) = −5·2 × 10 3 / T + 14·9 (2·0 M -Na + ) and log 10 k (min −1 ) = −5·0 × 10 3 / T + 13·6 (0·13 M -Na + ) can be used to estimate the rate of circle formation below 55 and 47°C, respectively." @default.
- W2044642956 created "2016-06-24" @default.
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- W2044642956 date "1966-01-01" @default.
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- W2044642956 title "Thermodynamic and kinetic studies on the interconversion between the linear and circular forms of phage lambda DNA" @default.
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- W2044642956 doi "https://doi.org/10.1016/s0022-2836(66)80213-9" @default.
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