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• W2044654702 abstract "Phosphoryl transfer, typically involving high energy phosphate donors such as ATP, is the most common class of biological reactions. Despite this, the transition state for phosphoryl transfer from ATP in solution has not been systematically investigated. Characterization of the transition state for the uncatalyzed hydrolysis of ATP would provide a starting point for dissection of enzyme-catalyzed reactions.We examined phosphoryl transfer from ATP, GTP and pyrophosphate to a series of alcohols; these reactions are analogous to the phosphorylation of sugars and other biological alcohols and to the hydrolysis of ATP. The Brønsted beta(nucleophile) value of 0.07 is small, indicating that there is little bond formation between the incoming nucleophile and the electrophilic phosphoryl group in the transition state. Coordination of Mg2+ has no measurable effect on this value. The Brønsted beta(leaving group) value of -1.1 for phosphoryl transfer to water from a series of phosphoanhydrides is large and negative, suggesting that the bond between phosphorous and the leaving group oxygen is largely broken in the transition state.Uncatalyzed hydrolysis of ATP in solution occurs via a dissociative, metaphosphate-like transition state, with little bond formation between nucleophile and ATP and substantial cleavage of the bond between the gamma-phosphoryl moiety and the ADP leaving group. Bound Mg2+ does not perturb the dissociative nature of the transition state, contrary to proposals that enzyme-bound metal ions alter this structure. The simplest expectation for phosphoryl transfer at the active site of enzymes thus entails a dissociative transition state. These results provide a basis for analyzing catalytic mechanisms for phosphoryl transfer." @default.
• W2044654702 created "2016-06-24" @default.
• W2044654702 creator A5034602076 @default.
• W2044654702 creator A5076625801 @default.
• W2044654702 date "1995-11-01" @default.
• W2044654702 modified "2023-10-18" @default.
• W2044654702 title "Mapping the transition state for ATP hydrolysis: implications for enzymatic catalysis" @default.
• W2044654702 cites W139107379 @default.
• W2044654702 cites W1570910952 @default.
• W2044654702 cites W1624188125 @default.
• W2044654702 cites W1821679912 @default.
• W2044654702 cites W1942192923 @default.
• W2044654702 cites W1964141556 @default.
• W2044654702 cites W1965695983 @default.
• W2044654702 cites W1969173610 @default.
• W2044654702 cites W1974857983 @default.
• W2044654702 cites W1976176097 @default.
• W2044654702 cites W1978737991 @default.
• W2044654702 cites W1982922463 @default.
• W2044654702 cites W1988269915 @default.
• W2044654702 cites W1989493014 @default.
• W2044654702 cites W1991945478 @default.
• W2044654702 cites W1991984236 @default.
• W2044654702 cites W1993466966 @default.
• W2044654702 cites W1993918760 @default.
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• W2044654702 cites W2015028401 @default.
• W2044654702 cites W2017225195 @default.
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• W2044654702 cites W2312610353 @default.
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• W2044654702 cites W2329418110 @default.
• W2044654702 cites W2331377560 @default.
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• W2044654702 doi "https://doi.org/10.1016/1074-5521(95)90101-9" @default.
• W2044654702 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9383480" @default.
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