FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2044720468> ?p ?o ?g. }

• W2044720468 endingPage "433" @default.
• W2044720468 startingPage "421" @default.
• W2044720468 abstract "A search for target proteins of inositol polyphosphates in mammalian tissues revealed that fructose 1,6-bisphosphate aldolases are potent isomer-selective binders of inositol polyphosphates. Binding was measured by tryptophan fluorescence quenching, by difference spectroscopy, and, in aldolase A, by equilibrium dialysis. Among a series of inositol phosphates containing between one and six phosphates and varying in their positions, inositol 1,4,5-trisphosphate was found to be bound strongest both by aldolase A ([L]0.5= 0.58 μM) and aldolase B ([L]0.5= 0.83 μM). Aldolase A showed also a strong binding of inositol tetrakisphosphate ([L]0.5= 0.83 μM), of insoitol 2,4,5-trisphosphate ([L]0.5= 1.4 μM) and of inositol 1,3,4,5,6-pentakisphosphate ([L]0.5= 2.0 μM); in aldolase B but not in aldolase A inositol 4,5-bisphosphate was bound as strongly as inositol 1,4,5-trisphosphate ([L]0.5= 0.95 μM) and also inositol 2,4,5-trisphosphate was tightly bound ([L]0.5= 1.2 μM). Both in aldolase A and B, 4 mol inositol 1,4,5-trisphosphate were bound/mol tetramer, in aldolase A a total binding of 8 mol inositol 1,4-bisphosphate/mol tetramer was evaluated. Difference spectra revealed that the binding of inositol phosphates to both isoenzymes may be associated with conformational changes. The binding of all inositol phosphates led to an inhibition of the enzyme activity. In aldolase A the inhibition was purely competitive, in aldolase B a complex cooperative type of inhibition was evident with fructose 1,6-bisphosphate as a substrate whereas with fructose 1-phosphate the inhibition also was purely competitive. Model calculations based on the in vitro data indicated a significant potential of aldolase to bind preferentially inositol 1,4,5-trisphosphate also in the presence of excess fructose 1,6-bisphosphate." @default.
• W2044720468 created "2016-06-24" @default.
• W2044720468 creator A5019350061 @default.
• W2044720468 creator A5032409973 @default.
• W2044720468 creator A5065557626 @default.
• W2044720468 date "1986-12-01" @default.
• W2044720468 modified "2023-09-26" @default.
• W2044720468 title "Mammalian aldolases are isomer-selective high-affinity inositol polyphosphate binders" @default.
• W2044720468 cites W1158770474 @default.
• W2044720468 cites W117990995 @default.
• W2044720468 cites W1495233477 @default.
• W2044720468 cites W1516035740 @default.
• W2044720468 cites W1535343987 @default.
• W2044720468 cites W1551134780 @default.
• W2044720468 cites W1554211606 @default.
• W2044720468 cites W1555778502 @default.
• W2044720468 cites W1557259171 @default.
• W2044720468 cites W1568068653 @default.
• W2044720468 cites W1669085278 @default.
• W2044720468 cites W1802980406 @default.
• W2044720468 cites W1904321711 @default.
• W2044720468 cites W1971035987 @default.
• W2044720468 cites W1978151819 @default.
• W2044720468 cites W1980732431 @default.
• W2044720468 cites W1992635174 @default.
• W2044720468 cites W2004325771 @default.
• W2044720468 cites W2004408288 @default.
• W2044720468 cites W2006731467 @default.
• W2044720468 cites W2007811793 @default.
• W2044720468 cites W2008195541 @default.
• W2044720468 cites W2008245136 @default.
• W2044720468 cites W2033041880 @default.
• W2044720468 cites W2049800254 @default.
• W2044720468 cites W2054716734 @default.
• W2044720468 cites W2057495669 @default.
• W2044720468 cites W2061503891 @default.
• W2044720468 cites W2066444129 @default.
• W2044720468 cites W2066691584 @default.
• W2044720468 cites W2068771093 @default.
• W2044720468 cites W2068877244 @default.
• W2044720468 cites W2078486370 @default.
• W2044720468 cites W2090546665 @default.
• W2044720468 cites W2092933559 @default.
• W2044720468 cites W2100087594 @default.
• W2044720468 cites W2103418001 @default.
• W2044720468 cites W2128635872 @default.
• W2044720468 cites W2201886059 @default.
• W2044720468 cites W2208268550 @default.
• W2044720468 cites W2263541816 @default.
• W2044720468 cites W2401644871 @default.
• W2044720468 cites W3021616478 @default.
• W2044720468 cites W303363201 @default.
• W2044720468 cites W4210975051 @default.
• W2044720468 cites W4233537042 @default.
• W2044720468 cites W978780368 @default.
• W2044720468 doi "https://doi.org/10.1111/j.1432-1033.1986.tb10462.x" @default.
• W2044720468 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3780751" @default.
• W2044720468 hasPublicationYear "1986" @default.
• W2044720468 type Work @default.
• W2044720468 sameAs 2044720468 @default.
• W2044720468 citedByCount "57" @default.
• W2044720468 countsByYear W20447204682013 @default.
• W2044720468 countsByYear W20447204682014 @default.
• W2044720468 countsByYear W20447204682017 @default.
• W2044720468 crossrefType "journal-article" @default.
• W2044720468 hasAuthorship W2044720468A5019350061 @default.
• W2044720468 hasAuthorship W2044720468A5032409973 @default.
• W2044720468 hasAuthorship W2044720468A5065557626 @default.
• W2044720468 hasBestOaLocation W20447204681 @default.
• W2044720468 hasConcept C170493617 @default.
• W2044720468 hasConcept C181199279 @default.
• W2044720468 hasConcept C184054246 @default.
• W2044720468 hasConcept C185592680 @default.
• W2044720468 hasConcept C2777427919 @default.
• W2044720468 hasConcept C2778886173 @default.
• W2044720468 hasConcept C28165981 @default.
• W2044720468 hasConcept C3488265 @default.
• W2044720468 hasConcept C55493867 @default.
• W2044720468 hasConcept C71240020 @default.
• W2044720468 hasConceptScore W2044720468C170493617 @default.
• W2044720468 hasConceptScore W2044720468C181199279 @default.
• W2044720468 hasConceptScore W2044720468C184054246 @default.
• W2044720468 hasConceptScore W2044720468C185592680 @default.
• W2044720468 hasConceptScore W2044720468C2777427919 @default.
• W2044720468 hasConceptScore W2044720468C2778886173 @default.
• W2044720468 hasConceptScore W2044720468C28165981 @default.
• W2044720468 hasConceptScore W2044720468C3488265 @default.
• W2044720468 hasConceptScore W2044720468C55493867 @default.
• W2044720468 hasConceptScore W2044720468C71240020 @default.
• W2044720468 hasIssue "2" @default.
• W2044720468 hasLocation W20447204681 @default.
• W2044720468 hasLocation W20447204682 @default.
• W2044720468 hasOpenAccess W2044720468 @default.
• W2044720468 hasPrimaryLocation W20447204681 @default.
• W2044720468 hasRelatedWork W1595482599 @default.
• W2044720468 hasRelatedWork W1942351213 @default.
• W2044720468 hasRelatedWork W1968993915 @default.
• W2044720468 hasRelatedWork W2003690817 @default.

• next