Matches in SemOpenAlex for { <https://semopenalex.org/work/W2044734498> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2044734498 endingPage "79" @default.
- W2044734498 startingPage "67" @default.
- W2044734498 abstract "In order to determine guanosine-5'-triphosphatase (GTPase) activity, we developed a simple, rapid and reliable method that utilizes capillary electrophoresis without radioisotope. Tubulin-GTPase was used for simple measurement of GTPase activity utilizing capillary electrophoresis. Tubulin, a component of microtubules, was incubated with guanosine-5'-triphosphate (GTP) in 100 mM 2-(N-morpholino) ethanesulfonic acid (MES) buffer (pH 6.5). Guanosine-5'-diphosphate (GDP) was determined as the hydrolyzed product of GTP. Guanosine-5'-monophosphate, GDP and GTP in the filtrate of the mixture were clearly separated using 10 mM MES buffer (pH 6.5) (migration time, 3.8, 5.5 and 7.2 minutes, respectively) with a fused-silica capillary column. The quantification of GDP was based on the peak area, which increased linearly with the concentration of GDP from 1 to 50 microM (r2=0.995). The peak area and migration time had good reproducibility; the intra-assay coefficient of variation (n=6) was 1.3% for peak area and 0.6% for migration time. As an application of this method, we examined the effect of dimethylarsinic acid, an effective antimitotic agent, on tubulin-GTPase. Dimethylarsinic acid inhibited tubulin-GTPase activity in a dose-dependent manner. The inhibition was not complete and the maximum decrease of the activity was about 50% at 200 microM dimethylarsinic acid. Thus, since this method is clean, simple and rapid, its application to the study of various GTPase proteins is expected to be useful." @default.
- W2044734498 created "2016-06-24" @default.
- W2044734498 creator A5007199938 @default.
- W2044734498 creator A5017332423 @default.
- W2044734498 creator A5018643143 @default.
- W2044734498 creator A5021633231 @default.
- W2044734498 creator A5066605454 @default.
- W2044734498 date "2000-01-01" @default.
- W2044734498 modified "2023-10-17" @default.
- W2044734498 title "Simple and Rapid Determination of GTPase Activity by Capillary Electrophoresis without Radioisotope" @default.
- W2044734498 cites W1969617066 @default.
- W2044734498 cites W1971007187 @default.
- W2044734498 cites W1974467854 @default.
- W2044734498 cites W1978992605 @default.
- W2044734498 cites W2009112465 @default.
- W2044734498 cites W2013020041 @default.
- W2044734498 cites W2028372527 @default.
- W2044734498 cites W2033998350 @default.
- W2044734498 cites W2038869813 @default.
- W2044734498 cites W2060705340 @default.
- W2044734498 cites W2071856833 @default.
- W2044734498 cites W2086578299 @default.
- W2044734498 cites W2092543552 @default.
- W2044734498 cites W2105405431 @default.
- W2044734498 cites W2128635872 @default.
- W2044734498 cites W2406738044 @default.
- W2044734498 cites W2409495339 @default.
- W2044734498 cites W246434313 @default.
- W2044734498 cites W2741522657 @default.
- W2044734498 doi "https://doi.org/10.1620/tjem.192.67" @default.
- W2044734498 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11128870" @default.
- W2044734498 hasPublicationYear "2000" @default.
- W2044734498 type Work @default.
- W2044734498 sameAs 2044734498 @default.
- W2044734498 citedByCount "6" @default.
- W2044734498 crossrefType "journal-article" @default.
- W2044734498 hasAuthorship W2044734498A5007199938 @default.
- W2044734498 hasAuthorship W2044734498A5017332423 @default.
- W2044734498 hasAuthorship W2044734498A5018643143 @default.
- W2044734498 hasAuthorship W2044734498A5021633231 @default.
- W2044734498 hasAuthorship W2044734498A5066605454 @default.
- W2044734498 hasBestOaLocation W20447344981 @default.
- W2044734498 hasConcept C118716 @default.
- W2044734498 hasConcept C181199279 @default.
- W2044734498 hasConcept C185592680 @default.
- W2044734498 hasConcept C20418707 @default.
- W2044734498 hasConcept C207332259 @default.
- W2044734498 hasConcept C2776638253 @default.
- W2044734498 hasConcept C2777995097 @default.
- W2044734498 hasConcept C2779340330 @default.
- W2044734498 hasConcept C2779817904 @default.
- W2044734498 hasConcept C2900643 @default.
- W2044734498 hasConcept C43617362 @default.
- W2044734498 hasConcept C55493867 @default.
- W2044734498 hasConcept C84425145 @default.
- W2044734498 hasConcept C86803240 @default.
- W2044734498 hasConcept C95444343 @default.
- W2044734498 hasConceptScore W2044734498C118716 @default.
- W2044734498 hasConceptScore W2044734498C181199279 @default.
- W2044734498 hasConceptScore W2044734498C185592680 @default.
- W2044734498 hasConceptScore W2044734498C20418707 @default.
- W2044734498 hasConceptScore W2044734498C207332259 @default.
- W2044734498 hasConceptScore W2044734498C2776638253 @default.
- W2044734498 hasConceptScore W2044734498C2777995097 @default.
- W2044734498 hasConceptScore W2044734498C2779340330 @default.
- W2044734498 hasConceptScore W2044734498C2779817904 @default.
- W2044734498 hasConceptScore W2044734498C2900643 @default.
- W2044734498 hasConceptScore W2044734498C43617362 @default.
- W2044734498 hasConceptScore W2044734498C55493867 @default.
- W2044734498 hasConceptScore W2044734498C84425145 @default.
- W2044734498 hasConceptScore W2044734498C86803240 @default.
- W2044734498 hasConceptScore W2044734498C95444343 @default.
- W2044734498 hasIssue "1" @default.
- W2044734498 hasLocation W20447344981 @default.
- W2044734498 hasLocation W20447344982 @default.
- W2044734498 hasOpenAccess W2044734498 @default.
- W2044734498 hasPrimaryLocation W20447344981 @default.
- W2044734498 hasRelatedWork W1965541346 @default.
- W2044734498 hasRelatedWork W1967664991 @default.
- W2044734498 hasRelatedWork W2011611048 @default.
- W2044734498 hasRelatedWork W2013883630 @default.
- W2044734498 hasRelatedWork W2044734498 @default.
- W2044734498 hasRelatedWork W2057337811 @default.
- W2044734498 hasRelatedWork W2061290720 @default.
- W2044734498 hasRelatedWork W2067149753 @default.
- W2044734498 hasRelatedWork W2090971051 @default.
- W2044734498 hasRelatedWork W2438117349 @default.
- W2044734498 hasVolume "192" @default.
- W2044734498 isParatext "false" @default.
- W2044734498 isRetracted "false" @default.
- W2044734498 magId "2044734498" @default.
- W2044734498 workType "article" @default.