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• W2044745091 abstract "It has been established that in a dilute solution individual giant DNA molecules undergo a large discrete transition between an elongated coil state and a folded compact state. On the other hand, in concentrated solutions, DNA molecules assemble into various characteristic states, including multichain aggregate, liquid crystalline, ionic crystal, etc. In this study, we compared single-chain and multiple-chain events by observing individual chains using fluorescence microscopy. We used spermidine, SPD(3+), as a condensing agent for giant DNA. When the concentration of DNA is below 1 μM in base-pair units, individual DNA molecules exhibit a transition from an elongated state to a compact state. When the concentration of DNA is increased to 10 μM, a thick fiberlike assembly of multiple chains appears. AFM measurements of this thick fiber revealed that more than tens of DNA molecules form a bundle structure with parallel ordering of the chains. The transition between single-chain compaction and bundle formation with multiple-chain assemblies was reproduced by a theoretical calculation." @default.
• W2044745091 created "2016-06-24" @default.
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• W2044745091 date "2004-02-20" @default.
• W2044745091 modified "2023-09-27" @default.
• W2044745091 title "Competition between compaction of single chains and bundling of multiple chains in giant DNA molecules" @default.
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• W2044745091 doi "https://doi.org/10.1063/1.1642610" @default.
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