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- W2044785669 abstract "Fluorescein isothiocyanate (FITC) was found to have a strong affinity to Toyopearl gel, which is used for gel filtration. FITC-labelled amino acids also showed affinity to Toyopearl gel, their elution from a Toyopearl HW-40 column being retarded. On the other hand, FITC-labelled proteins had no affinity to the gel and were recovered in the flow-through fractions. These findings were applied to the analysis of the degradation of various FITC-labelled proteins by lysosomal enzymes in vitro. FITC-labelled degradation products were easily separated from FITC-labelled substrate proteins on a small Toyopearl HW-40 column. Their production increased with the incubation time and was markedly suppressed by the proteinase inhibitor leupeptin. The FITC-labelled degradation product was identified to be mainly lysine with a FITC-labelled epsilon-amino group by its different elution position to those of lysine with a FITC-labelled alpha-amino group and other various FITC-labelled amino acids." @default.
- W2044785669 created "2016-06-24" @default.
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- W2044785669 date "1993-02-01" @default.
- W2044785669 modified "2023-10-14" @default.
- W2044785669 title "Analysis of lysosomal degradation of fluorescein isothiocyanate-labelled proteins by Toyopearl HW-40 affinity chromatography" @default.
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- W2044785669 doi "https://doi.org/10.1016/0021-9673(93)83164-n" @default.
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