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- W2044797535 abstract "1. The preparation of chloroplasts and chloroplast fragments from swiss-chard, which were active in photophosphorylation is described. A chloroplast preparation stable for 24–48 h could be obtained. 2. A sensitive assay for the ATP formed was adopted. It allowed the measurement of very small amounts of ATP formed, and avoided several difficulties encountered in other assays. 3. The requirements in the homogenizing medium and in the reaction mixture were studied. Factors studied included: ascorbate, gas phase, pH, cofactors, magnesium, phosphate, nucleotide specificity and light intensity. 4. The chloroplasts could phosphorylate ADP, GDP, or IDP at high rates, UDP at low rates, and CDP not at all. Nucleoside diphosphokinase(s) in the preparations was demonstrated. 5. No adenyl kinase activity could be found in washed or fragmented swiss-chard chloroplasts. 6. Rates of 2000–2500 μmoles ATP formed/mg chlorophyll/h were obtained under optimal conditions, which included low chloroplast concentrations, short reaction times, appropriate ascorbate concentration, and very high light intensities (approx. 150,000 lux). 7. Confirmation was presented for the ineffectiveness of 2,4-dinitrophenol and effectiveness of ammonium ions as uncoupling agents, and the lack of exchange between inorganic phosphate and ATP in swiss-chard chloroplasts. 8. No exchange between ADP and ATP could be demonstrated." @default.
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- W2044797535 date "1960-01-01" @default.
- W2044797535 modified "2023-10-06" @default.
- W2044797535 title "Photophosphorylation by swiss-chard chloroplasts" @default.
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- W2044797535 doi "https://doi.org/10.1016/0006-3002(60)91350-0" @default.
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