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- W2044803462 abstract "The deproteinization of human plasma was carried out using a hydroxy apatite cartridge as a precolumn. After human plasma had been passed through the cartridge followed by suitable elution, protein-free eluate was obtained within only 1 min without the need for centrifugation. Deproteinization was evaluated by the determination of albumin, γ-globulin and transferrin in the eluate by high-performance liquid chromatography (HPLC) with UV detection. Determination of theophylline and diazepam in human plasma was performed by HPLC with UV detection. The proposed method was suitable for the determination of these two drugs in human plasma, because it is simple and rapid (retention time of each drugs ≈ 15 min) and microamounts of sample are required (50–100 μl). The calibration graphs for theophylline and diazepam were linear in the range 0.1–10 μg and 0.1–65 ng, respectively. Recoveries of both drugs were over 90% by the standard addition method." @default.
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- W2044803462 date "1994-04-01" @default.
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- W2044803462 title "Novel precolumn deproteinization method using a hydroxyapatite cartridge for the determination of theophylline and diazepam in human plasma by high-performance liquid chromatography with ultraviolet detection" @default.
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- W2044803462 doi "https://doi.org/10.1016/0378-4347(94)00055-7" @default.
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