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• W2044852031 endingPage "106" @default.
• W2044852031 startingPage "93" @default.
• W2044852031 abstract "λ DNA as in vitro packaging substrate can either be a linear monomer with λ cohesive ends or a concatemer with at least two sites of cohered ends, in which case the sites are cleaved during packaging. Packaging is initiated by complexing of either monomeric or concatemeric DNA with the phage-specific A protein, the protein also responsible for the DNA cutting. This is demonstrated by the fact that during in vitro packaging DNA in an A+ lysate differs from DNA in an A− lysate or from exogenously added λ DNA in its requirement for A protein and in its packaging kinetics. The formation of this A protein-DNA complex seems to require a specific site or sites on the DNA. A protein as measured by its function to build this complex and to cleave the DNA does not turn over." @default.
• W2044852031 created "2016-06-24" @default.
• W2044852031 creator A5041039521 @default.
• W2044852031 date "1975-10-01" @default.
• W2044852031 modified "2023-10-16" @default.
• W2044852031 title "DNA as substrate for packaging into bacteriophage lambda, in vitro" @default.
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• W2044852031 doi "https://doi.org/10.1016/s0022-2836(75)80103-3" @default.
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• W2044852031 hasPublicationYear "1975" @default.
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