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• W2044889035 abstract "Recently, synthetic prions with a high level of specific infectivity have been produced from chemically defined components in vitro. A major insight arising from these studies is that various classes of host-encoded cofactor molecules such as phosphatidylethanolamine and RNA molecules are required to form and maintain the specific conformation of infectious prions. Synthetic mouse prions formed with phosphatidylethanolamine exhibit levels of specific infectivity ∼1 million-fold greater than protein-only prions (Deleault, N. R., Walsh, D. J., Piro, J. R., Wang, F., Wang, X., Ma, J., Rees, J. R., and Supattapone, S. (2012) Proc. Natl. Acad. Sci. U.S.A. 109, E1938-E1946). Moreover, cofactor molecules also appear to regulate prion strain properties by limiting the potential conformations of the prion protein (see Deleault et al. above). The production of fully infectious synthetic prions provides new opportunities to study the mechanism of prion infectivity directly by structural and biochemical methods." @default.
• W2044889035 created "2016-06-24" @default.
• W2044889035 creator A5016987747 @default.
• W2044889035 date "2014-07-01" @default.
• W2044889035 modified "2023-10-12" @default.
• W2044889035 title "Synthesis of High Titer Infectious Prions with Cofactor Molecules" @default.
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• W2044889035 doi "https://doi.org/10.1074/jbc.r113.511329" @default.
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