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• W2044895851 startingPage "412" @default.
• W2044895851 abstract "Bacterial contamination of blood components, especially of platelet concentrates (PCs), represents the highest infectious risk in blood transfusion. Although the incidence of platelet bacterial contamination is approximately one per 2000 units, the urgent need for a method for the routine screening of PCs to improve safety for patients had not been considered for a long time. Besides the culturing systems, which will remain the gold standard, rapid methods for sterility screening will play a more important role in transfusion medicine in the future. In particular, the nucleic acid amplification techniques (NAT) are powerful tools in bacterial screening assays. The combination of excellent sensitivity and specificity, low contamination risk, ease of performance and speed, has made real-time PCR technology an appealing alternative to conventional culture-based testing methods. When using real-time PCR for the detection of bacterial contamination, several points have to be considered. The main focus refers to the choice of the target gene, the assay format, the nucleic acid extraction method, depending on the sample type, and the evaluation of an ideal sampling strategy. However, several factors like the bacterial-derived nucleic acid contamination of amplification reagents or laboratory materials, the impracticability and the costs have limited the use of NAT until now. Attempts to reduce the amount of contaminating nucleic-acids from reagents in real-time PCR have been described, but none of these methods have proved to be very effective or may lower the sensitivity of the assay. Recently, a number of broad-range NAT assays targeting the 16S rDNA or 23S rRNA for detection of bacteria based on real-time technology have been reported. This review will give a short survey of current approaches to and limitations of the application of real-time PCR for bacterial detection in blood components, with emphasis on PCs." @default.
• W2044895851 created "2016-06-24" @default.
• W2044895851 creator A5064472853 @default.
• W2044895851 creator A5068483451 @default.
• W2044895851 creator A5073817989 @default.
• W2044895851 date "2006-12-01" @default.
• W2044895851 modified "2023-09-26" @default.
• W2044895851 title "Real-time NAT-based methods for detection of bacterial contamination in blood products / Real-time NAT-basierte Methoden zur Entdeckung bakterieller Kontamination in Blutprodukten" @default.
• W2044895851 cites W1484113940 @default.
• W2044895851 cites W152703982 @default.
• W2044895851 cites W1596499576 @default.
• W2044895851 cites W1791412297 @default.
• W2044895851 cites W1852890074 @default.
• W2044895851 cites W1913892813 @default.
• W2044895851 cites W1932767033 @default.
• W2044895851 cites W1960080934 @default.
• W2044895851 cites W1965148687 @default.
• W2044895851 cites W1966637572 @default.
• W2044895851 cites W1967818087 @default.
• W2044895851 cites W1968177952 @default.
• W2044895851 cites W1970952326 @default.
• W2044895851 cites W1971952038 @default.
• W2044895851 cites W1987176207 @default.
• W2044895851 cites W1991530893 @default.
• W2044895851 cites W1992076170 @default.
• W2044895851 cites W1992116183 @default.
• W2044895851 cites W1992661590 @default.
• W2044895851 cites W1995656159 @default.
• W2044895851 cites W1997504088 @default.
• W2044895851 cites W2005366972 @default.
• W2044895851 cites W2009277397 @default.
• W2044895851 cites W2009964726 @default.
• W2044895851 cites W2027207787 @default.
• W2044895851 cites W2027839087 @default.
• W2044895851 cites W2029857713 @default.
• W2044895851 cites W2030729461 @default.
• W2044895851 cites W2032415319 @default.
• W2044895851 cites W2033381555 @default.
• W2044895851 cites W2040499564 @default.
• W2044895851 cites W2043807816 @default.
• W2044895851 cites W2044368974 @default.
• W2044895851 cites W2047737699 @default.
• W2044895851 cites W2049767543 @default.
• W2044895851 cites W2049890674 @default.
• W2044895851 cites W2050839223 @default.
• W2044895851 cites W2051900180 @default.
• W2044895851 cites W2059348499 @default.
• W2044895851 cites W2059579719 @default.
• W2044895851 cites W2062828837 @default.
• W2044895851 cites W2068547146 @default.
• W2044895851 cites W2069797500 @default.
• W2044895851 cites W2070801207 @default.
• W2044895851 cites W2070871769 @default.
• W2044895851 cites W2073079522 @default.
• W2044895851 cites W2073393199 @default.
• W2044895851 cites W2080081235 @default.
• W2044895851 cites W2080454873 @default.
• W2044895851 cites W2081411589 @default.
• W2044895851 cites W2090268939 @default.
• W2044895851 cites W2091740417 @default.
• W2044895851 cites W2098666592 @default.
• W2044895851 cites W2099368476 @default.
• W2044895851 cites W2102612854 @default.
• W2044895851 cites W2106057103 @default.
• W2044895851 cites W2108928365 @default.
• W2044895851 cites W2111949763 @default.
• W2044895851 cites W2113749787 @default.
• W2044895851 cites W2114315172 @default.
• W2044895851 cites W2118830176 @default.
• W2044895851 cites W2119107074 @default.
• W2044895851 cites W2121923279 @default.
• W2044895851 cites W2123517420 @default.
• W2044895851 cites W2123823902 @default.
• W2044895851 cites W2125420735 @default.
• W2044895851 cites W2126768169 @default.
• W2044895851 cites W2129275226 @default.
• W2044895851 cites W2132986621 @default.
• W2044895851 cites W2133245784 @default.
• W2044895851 cites W2134103927 @default.
• W2044895851 cites W2134430146 @default.
• W2044895851 cites W2134440848 @default.
• W2044895851 cites W2134721999 @default.
• W2044895851 cites W2139205857 @default.
• W2044895851 cites W2142190378 @default.
• W2044895851 cites W2143706002 @default.
• W2044895851 cites W2144080673 @default.
• W2044895851 cites W2144677167 @default.
• W2044895851 cites W2145599304 @default.
• W2044895851 cites W2148682495 @default.
• W2044895851 cites W2150905188 @default.
• W2044895851 cites W2151119271 @default.
• W2044895851 cites W2154401636 @default.
• W2044895851 cites W2155493680 @default.
• W2044895851 cites W2156667451 @default.
• W2044895851 cites W2157584041 @default.
• W2044895851 cites W2157871174 @default.
• W2044895851 cites W2158894328 @default.
• W2044895851 cites W2161199707 @default.

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