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• W2044897481 abstract "Human immunodeficiency virus type 1 (HIV-1) genetic diversity presents a challenge to nucleic acid-based assays with regard to sensitivity of detection and accuracy of quantification. The Abbott LCx HIV RNA Quantitative assay (LCx(R) HIV assay), a competitive RT-PCR targeting the pol integrase region, was evaluated using a panel of 297 HIV-1 seropositive plasma samples from Cameroon, Uganda, Brazil, Thailand, Spain, Argentina and South Africa. The panel included group M subtypes A-G, mosaics, and group O based on sequence analysis of gag p24, pol integrase, and env gp41. The LCx HIV assay quantified 290 (97.6%) of the samples, including all the group O samples tested. In comparison, the Roche AMPLICOR HIV-1 MONITOR test versions 1.0 and 1.5 quantified 67.3 and 94.6% of the samples, respectively. No group O specimens were quantified by either version of AMPLICOR HIV-1 MONITOR. Seven specimens were below the detectable limits of all the three assays. The LCx HIV assay had fewer nucleotide mismatches at primer/probe binding sites as compared with both AMPLICOR HIV-1 MONITOR tests. The high degree of nucleotide conservation within the pol target region enables the LCx HIV assay to efficiently quantify the HIV-1 subtypes A-G and the most genetically diverse HIV-1, group O." @default.
• W2044897481 created "2016-06-24" @default.
• W2044897481 creator A5000935925 @default.
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• W2044897481 date "2000-09-01" @default.
• W2044897481 modified "2023-10-16" @default.
• W2044897481 title "Quantification of HIV-1 group M (subtypes A–G) and group O by the LCx HIV RNA quantitative assay" @default.
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• W2044897481 doi "https://doi.org/10.1016/s0166-0934(00)00205-6" @default.
• W2044897481 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10996643" @default.
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