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- W2044933014 abstract "Factor D is an essential enzyme for activation of complement by the alternative pathways (AP). It has been difficult to obtain mouse monoclonal antibodies (Mabs) which block the function of factor D. We have developed a strategy to obtain Mabs using a double screening procedure of the initial clones. We selected the clone whose supernatant had the lowest level of anti-factor D Ab by ELISA and abolished factor D haemolytic activity. Addition of this Mab to human serum was shown to abolish conversion of C2 cobra venom factor, haemolysis of rabbit erytrocytes, and activation of Ce and C5 by cuprophane dialysis membranes." @default.
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- W2044933014 date "1990-03-01" @default.
- W2044933014 modified "2023-09-27" @default.
- W2044933014 title "A monoclonal antibody which blocks the function of factor D of human complement" @default.
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- W2044933014 doi "https://doi.org/10.1016/0022-1759(90)90077-9" @default.
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