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• W2044974411 abstract "Abstract The response of animals adapted to moderate or severe energy restriction to supplements of free amino acids or protein is unknown. Lysine supplementation was examined because it is an amino acid frequently limiting in cereal-based diets. Male rats weighing 115 g were assigned to one of seven diet groups. Animals were fed either a gluten-based diet or the gluten diet supplemented with adequate levels of lysine for 8 weeks. These diets were fed ad libitum or at 40% or 75% restricted levels. An additional group was fed a high gluten diet at the same energy level as the 75% restricted rats. Body weight, muscle weights, and adipose tissue were reduced relative to the level of restriction. Restricted groups adapted to the energy restrictions and exhibited either slow growth or stable body weight after 4 weeks on the respective treatments. Lysine supplementation increased muscle mass and improved nitrogen balance in the ad libitum and 40% restricted groups, but had no effect on the 75% restricted group. Excretion of 3-methylhistidine (3MH) was higher in the energy restricted animals after 7 weeks on the respective diets, and lysine supplements further increased 3MH losses. Plasma lysine levels were reduced by the food restrictions and unaffected by lysine supplements in the restricted animals. These findings indicate that the efficacy of lysine supplementation depends on the absolute level of energy intake, and a lysine supplemented diet may be detrimental to skeletal muscle mass at very low energy intakes. The data also showed that rats adapt to a 75% food restriction by achieving and maintaining nitrogen balance despite the increase in myofibrillar protein breakdown." @default.
• W2044974411 created "2016-06-24" @default.
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• W2044974411 date "1993-09-01" @default.
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• W2044974411 title "Nitrogen metabolism at chronic low energy intakes" @default.
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• W2044974411 doi "https://doi.org/10.1016/s0271-5317(05)80524-x" @default.
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