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- W2044986989 abstract "The purpose of this study was to evaluate the effect of cooling ovarian tissue on pig pre-antral follicles. Ovaries were maintained in saline solution (0.9%) at 4 or 20°C for 6, 12 or 18 h. After storage, pre-antral follicles were morphologically evaluated. While primordial follicles were not affected by the storage, the percentage of morphologically normal growing follicles was significantly reduced in ovarian tissue stored at 20°C for 12 or 18 h. To test the viability of stored follicles, growing follicles isolated from ovaries stored at 4°C for 18 h and at 20°C for 6 h were cultured for 3 days. Follicles stored in either condition presented the same growth pattern in vitro as fresh follicles. We conclude that storage of pig ovaries at 4°C for up to 18 h or at 20°C for up to 6 h does not affect the morphology of growing follicles or their ability to grow in vitro." @default.
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- W2044986989 date "2006-09-21" @default.
- W2044986989 modified "2023-09-25" @default.
- W2044986989 title "Effects of Storing Pig Ovaries at 4 or 20°C for Different Periods of Time on the Morphology and Viability of Pre-Antral Follicles" @default.
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- W2044986989 doi "https://doi.org/10.1111/j.1439-0531.2006.00734.x" @default.
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