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• W2044989850 endingPage "1381" @default.
• W2044989850 startingPage "1373" @default.
• W2044989850 abstract "Inhaled nanoparticles have been reported to contribute to deleterious effects on human health. In this study, we investigated the effects of ultrafine ambient particulate suspensions (UAPS), polystyrene nanoparticles (PNP; positively and negatively charged; 20, 100, 120 nm), quantum dots (QD; positively and negatively charged; 30 nm) and single-wall carbon nanotubes (SWCNT) on alveolar epithelial cell barrier properties. Transmonolayer resistance (Rt) and equivalent short-circuit current (Ieq) of primary rat alveolar epithelial monolayers were measured in the presence and absence of varying concentrations of apical nanoparticles. In some experiments, apical-to-basolateral fluxes of radiolabeled mannitol or inulin were determined with or without apical UAPS exposure and lactate dehydrogenase (LDH) release was analyzed after UAPS or SWCNT exposure. Results revealed that exposure to UAPS decreased Rt and Ieq significantly over 24 h, although neither mannitol nor inulin fluxes changed. Positively charged QD decreased Rt significantly (with subsequent recovery), while negatively charged QD did not. Rt decreased significantly after SWCNT exposure (with subsequent recovery). On the other hand, PNP exposure had no effects on Rt or Ieq. No significant increases in LDH release were observed after UAPS or SWCNT exposure. These data indicate that disruption of alveolar epithelial barrier properties due to apical nanoparticle exposure likely involves alteration of cellular transport pathways and is dependent on specific nanoparticle composition, shape and/or surface charge." @default.
• W2044989850 created "2016-06-24" @default.
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• W2044989850 date "2007-12-01" @default.
• W2044989850 modified "2023-10-12" @default.
• W2044989850 title "Nanoparticle effects on rat alveolar epithelial cell monolayer barrier properties" @default.
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• W2044989850 doi "https://doi.org/10.1016/j.tiv.2007.04.003" @default.
• W2044989850 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3855017" @default.
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• W2044989850 hasPublicationYear "2007" @default.
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