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- W2045002571 abstract "In the present study, the optimization of production and reaction conditions of polygalacturonase produced by a fungus Byssochlamys fulva MTCC 505 was achieved. The production of polygalacturonase with a considerable activity of 1.28 IU/ml was found when the culture was shaken at 30°C for 5 days in 100 ml of medium containing (w/v) 10 g/l pectin, 2 g/l NaNO 3 , 1 g/l KH 2 PO 4 , 0.5 g/l KCl, 0.5 g/l MgSO 4 . 7H 2 O, 0.001 g/l FeSO 4 . 7H 2 O, 0.001 g/l CaCl 2 . The best carbon and nitrogen source for this enzyme were pectin (1%) and Ca(NO 3 ) 2 (0.1%), respectively. The enzyme gave maximum activity at incubation time of 72 h, temperature of 30°C and pH 4.5. During the optimization of reaction conditions, the enzyme showed maximum activity in sodium citrate buffer (50 mM) of pH 5.5 at 50°C reaction temperature for 15 minutes of incubation. The enzyme showed greater affinity for polygalacturonic acid as substrate (0.5%). K m and V max values were 0.15 mg/ml and 4.58 μmol/ml/min. The effect of various phenolics, thiols, protein inhibitors and metal ions on the enzyme activity was investigated. The enzyme was quite stable at 4°C and 30°C. At 40°C the half life of the enzyme was 6 h and at 60°C it was 2 h." @default.
- W2045002571 created "2016-06-24" @default.
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- W2045002571 date "2011-12-01" @default.
- W2045002571 modified "2023-09-23" @default.
- W2045002571 title "Optimization of production and reaction conditions of polygalacturonase from Byssochlamys fulva" @default.
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- W2045002571 doi "https://doi.org/10.1556/amicr.58.2011.4.9" @default.
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