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• W2045117922 endingPage "321" @default.
• W2045117922 startingPage "315" @default.
• W2045117922 abstract "The Feulgen reaction and the staining of preparations with two DNA-specific fluorochromes, Hoechst 33258 and 4′,6-diamidino-2-fenilindol (DAPI), were used to study the preservation of DNA in the fossilized leaf and fruit tissues of the Lower Eocene Myrtaceae, Paramyrtacicarpus plurilocularis and Paramyrtaciphyllum agapovii collected in Yakutia (Siberia, Russia). It was shown that chromatin structures of the fossilized plants form stable red-purple complexes with the Schiff's fuchsin sulphuric acid reagent in situ. This coloration is specific for DNA, in particular, for the deoxyribose residues. It means that the cell nuclei of these 53–55 Myr old plants preserve a part of the deoxyribose backbone of DNA molecules. On the other hand, there was no, or only a very weak, staining of the cell nucleus with fluorochromes DAPI or Hoechst 33258, which specifically bind to the double-stranded DNA and do not bind to either the single-stranded DNA or RNA molecules. The stainability of fossil plant cell preparations with alcian blue shows that there are also polysaccharides containing carboxyl groups in the cell walls of fossilized leaf and fruit tissues of the Lower Eocene Myrtaceae." @default.
• W2045117922 created "2016-06-24" @default.
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• W2045117922 date "2006-03-01" @default.
• W2045117922 modified "2023-09-25" @default.
• W2045117922 title "Feulgen-positive staining of the cell nuclei in fossilized leaf and fruit tissues of the Lower Eocene Myrtaceae" @default.
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• W2045117922 doi "https://doi.org/10.1111/j.1095-8339.2006.00471.x" @default.
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