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- W2045124886 abstract "To solve the problem of fixed dried blood spot elution without damaging IgG antibodies.The minimum effective concentration of liquid ammonium (NH3 ) in a PBS solution, which was found to elute fixed blood, was determined. By using a dilution series, the effects of NH3 on IgG antibody quality were assessed using immunofluorescence assays.The minimum effective concentration of 0.2% NH3 has no detectable effects on IgG quality.Ammonium greatly improves blood elution from fixed DBS while maintaining IgG antibody quality. These results are encouraging and provide a basis for further testing of the efficacy of ammonium in different situations as well as its effect on other compounds." @default.
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- W2045124886 date "2014-01-06" @default.
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- W2045124886 title "Ammonium improves elution of fixed dried blood spots without affecting immunofluorescence assay quality" @default.
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- W2045124886 doi "https://doi.org/10.1111/tmi.12259" @default.
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