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- W2045130114 abstract "(1) Age-dependent changes of the pattern of sulfated glycosaminoglycan excretion in urine were investigated by electrophoresis on cellulose acetate membrane in 0.05 M barium acetate buffer. Clear separation between sulfated glycosaminoglycans could be achieved by this method. (2) The relative proportion of urinary glycosaminoglycans resistant to testicular hyaluronidase or to chondroitinase ABC increased with advancing age. More precisely, the increase in the excretion of the materials resistant to chondroitinase ABC was characteristic of aged individuals. (3) Urinary glycosaminoglycans resistant to testicular hyaluronidase were separable into two fractions by electrophoresis or ion-exchange column chromatography. Chemical analysis and enzymatic susceptibility of these materials suggest that these two fractions might be composed of heparan sulfate and dermatan sulfate, respectively. (4) The electrophoretic patterns of glycosamonoglycans in Hurler-Hunter's urine were quite different from that of normal urine. The relative proportions of dermatan sulfate, heparan sulfate and chondroitin sulfate A/C were important for the differentiation between Hurler and Hunter syndrome." @default.
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- W2045130114 title "Age differences in the pattern of urinary glycosaminoglycan excretion in normal individuals" @default.
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- W2045130114 doi "https://doi.org/10.1016/0009-8981(72)90437-8" @default.
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