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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045131013> ?p ?o ?g. }

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• W2045131013 startingPage "237" @default.
• W2045131013 abstract "A low cost, 0.75-mW helium neon laser, operating in the green region at 534.5 nm, is used to excite fluorescence from tetramethylrhodamine isothiocyanate-labelled DNA fragments that have been separated by capillary gel electrophoresis. The detection limit (3 sigma) for the dye is 500 ymol [1 yoctomole (1 ymol) = 10(-24) mol] or 300 analyte molecules in capillary zone electrophoresis; the detection limit for labeled primer separated by capillary gel electrophoresis is 2 zmol [1 zeptomole (1 zmol) = 10(-21) mol]. The Richardson-Tabor peak-height encoded sequencing technique is used to prepare DNA sequencing samples. In 6% T, 5% C acrylamide, 7 M urea gels, sequencing rates of 300 bases/hour are produced at an electric field strength of 200 V/cm; unfortunately, the data are plagued by compressions. These compressions are eliminated with addition of 20% formamide to the sequencing gel; the gel runs slowly and sequencing data are generated at a rate of about 70 bases/hour." @default.
• W2045131013 created "2016-06-24" @default.
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• W2045131013 date "1991-10-01" @default.
• W2045131013 modified "2023-10-10" @default.
• W2045131013 title "Low-cost, high-sensitivity laser-induced fluorescence detection for DNA sequencing by capillary gel electrophoresis" @default.
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• W2045131013 doi "https://doi.org/10.1016/0021-9673(91)80074-q" @default.
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