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- W2045138060 abstract "Liver from Squalus acanthias (spiny dogfish), a representative elasmobranch, contains ≈1.4 units (μmol/ min) of phosphoenolpyruvate carboxykinase activity per gram and ≈90% of the total units of activity are localized in the mitochondria. The mitochondrial phosphoenolpyruvate carboxykinase was isolated and characterized. The purified enzyme has properties generally similar to those found in mammalian and avian species. The enzyme has a molecular weight of ≈70,000 and exists in a functional state as a monomer. The isolated enzyme displays a dual cation requirement (e.g., 6 mm Mg2+ and 10 μm Mn2+) for maximal activity; very little activity is observed when Mg2+ is present alone, and the maximal activity attained with Mn2+ alone (millimolar concentrations required) is significantly less than that observed under optimal conditions with both cations present. When assayed in the direction of oxalacetate formation there is a lag in product formation with time; the lag can be eliminated by the presence of 50 μm GTP (product). The Km for substrates is not affected by Mn2+ concentration, suggesting that the role of Mn2+ may not be related to substrate binding. The apparent Km for phosphoenolpyruvate (≈1 mm) is substantially higher than that reported for phosphoenolpyruvate carboxykinase from other species. The activity of phosphoenolpyruvate carboxykinase is increased 70% by physiological concentrations of urea. Maximal velocity of the reaction in the direction of oxalacetate formation is approximately half that of the reverse reaction." @default.
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- W2045138060 title "Purification and properties of mitochondrial phosphoenolpyruvate carboxykinase from liver of Squalus acanthias" @default.
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- W2045138060 doi "https://doi.org/10.1016/0003-9861(90)90345-y" @default.
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