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• W2045162913 abstract "CD45RA has been found highly expressed on leukemia cells and may be a potential target of the disease. In this study, an anti-CD45RA single-chain antibody fragment (scFv3A4) was genetically linked to the N terminus of the enhanced green fluorescent protein (EGFP) to generate a scFv3A4–EGFP fusion protein. The scFv3A4–EGFP with a molecular weight of 57 kDa was stably expressed and secreted from the transfected CHO cells through the ER/Golgi-dependent pathway. The fusion protein was soluble in the culture supernatant and the yield was 1350 μg/L. Flow cytometry analysis showed that the scFv3A4–EGFP had the same binding site and a very similar reactivity pattern with its parental murine monoclonal antibody (mAb) 3A4. Furthermore, comparing to conventional labeled 3A4-FITC antibody, the scFv3A4–EGFP was more resistant to illumination and more suitable for immunofluorescence histology (IFH) detection. Therefore, the scFv3A4–EGFP fusion protein can be a powerful tool to investigate the targeting of CD45RA on leukemia cells, biological activity of the target and possibly for the genetic manipulation of the antibody." @default.
• W2045162913 created "2016-06-24" @default.
• W2045162913 creator A5023308874 @default.
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• W2045162913 date "2014-02-01" @default.
• W2045162913 modified "2023-09-24" @default.
• W2045162913 title "Successful construction and stable expression of an anti-CD45RA scFv–EGFP fusion protein in Chinese hamster ovary cells" @default.
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• W2045162913 doi "https://doi.org/10.1016/j.pep.2013.10.017" @default.
• W2045162913 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24211776" @default.
• W2045162913 hasPublicationYear "2014" @default.
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