Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045164101> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2045164101 endingPage "12" @default.
- W2045164101 startingPage "3" @default.
- W2045164101 abstract "Carboxypeptidase-A and a monoclonal antibody (KS1/4) directed against a human lung carcinoma cell line (UCLA-P3) were derivatized by treatment with succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate and N-succinimidyl 3-(2-pyridyldithio)propionate, respectively. Admixture of these entities produced a stable conjugate containing 4 to 5 enzyme molecules per molecule of antibody. The conjugate (Mr ≅ 300 kDa) was purified to homogeneity by HPLC gel filtration and HPLC ion-exchange chromatography. Neither the catalytic activity of the enzyme nor the antigen-binding capacity of the monoclonal antibody was impaired in the conjugate. UCLA-P3 cells that had been exposed to the conjugate and then washed thoroughly were extremely sensitive to methotrexate α-alanine (MTX-Ala), a prodrug form of MTX. At 10−5m, MTX-Ala was almost as effective as free MTX in blocking the replication of conjugate-treated cells. These results demonstrate the chemotherapeutic potential of enzyme-monoclonal antibody conjugates used in conjunction with prodrugs." @default.
- W2045164101 created "2016-06-24" @default.
- W2045164101 creator A5030778521 @default.
- W2045164101 creator A5036525889 @default.
- W2045164101 creator A5059305386 @default.
- W2045164101 creator A5073685371 @default.
- W2045164101 creator A5089432735 @default.
- W2045164101 date "1991-01-01" @default.
- W2045164101 modified "2023-10-14" @default.
- W2045164101 title "Construction and chemotherapeutic potential of carboxypeptidase-A/monoclonal antibody conjugate" @default.
- W2045164101 cites W1811830539 @default.
- W2045164101 cites W1977142690 @default.
- W2045164101 cites W1983430641 @default.
- W2045164101 cites W2054542253 @default.
- W2045164101 cites W2058093571 @default.
- W2045164101 cites W2071954948 @default.
- W2045164101 cites W2106239871 @default.
- W2045164101 cites W2109689528 @default.
- W2045164101 cites W2418948044 @default.
- W2045164101 doi "https://doi.org/10.1016/0065-2571(91)90005-7" @default.
- W2045164101 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1877392" @default.
- W2045164101 hasPublicationYear "1991" @default.
- W2045164101 type Work @default.
- W2045164101 sameAs 2045164101 @default.
- W2045164101 citedByCount "18" @default.
- W2045164101 countsByYear W20451641012013 @default.
- W2045164101 crossrefType "journal-article" @default.
- W2045164101 hasAuthorship W2045164101A5030778521 @default.
- W2045164101 hasAuthorship W2045164101A5036525889 @default.
- W2045164101 hasAuthorship W2045164101A5059305386 @default.
- W2045164101 hasAuthorship W2045164101A5073685371 @default.
- W2045164101 hasAuthorship W2045164101A5089432735 @default.
- W2045164101 hasConcept C108215921 @default.
- W2045164101 hasConcept C108477463 @default.
- W2045164101 hasConcept C134306372 @default.
- W2045164101 hasConcept C153911025 @default.
- W2045164101 hasConcept C159654299 @default.
- W2045164101 hasConcept C181199279 @default.
- W2045164101 hasConcept C185592680 @default.
- W2045164101 hasConcept C197336794 @default.
- W2045164101 hasConcept C203014093 @default.
- W2045164101 hasConcept C2778556491 @default.
- W2045164101 hasConcept C33923547 @default.
- W2045164101 hasConcept C542903549 @default.
- W2045164101 hasConcept C55493867 @default.
- W2045164101 hasConcept C86803240 @default.
- W2045164101 hasConceptScore W2045164101C108215921 @default.
- W2045164101 hasConceptScore W2045164101C108477463 @default.
- W2045164101 hasConceptScore W2045164101C134306372 @default.
- W2045164101 hasConceptScore W2045164101C153911025 @default.
- W2045164101 hasConceptScore W2045164101C159654299 @default.
- W2045164101 hasConceptScore W2045164101C181199279 @default.
- W2045164101 hasConceptScore W2045164101C185592680 @default.
- W2045164101 hasConceptScore W2045164101C197336794 @default.
- W2045164101 hasConceptScore W2045164101C203014093 @default.
- W2045164101 hasConceptScore W2045164101C2778556491 @default.
- W2045164101 hasConceptScore W2045164101C33923547 @default.
- W2045164101 hasConceptScore W2045164101C542903549 @default.
- W2045164101 hasConceptScore W2045164101C55493867 @default.
- W2045164101 hasConceptScore W2045164101C86803240 @default.
- W2045164101 hasLocation W20451641011 @default.
- W2045164101 hasLocation W20451641012 @default.
- W2045164101 hasOpenAccess W2045164101 @default.
- W2045164101 hasPrimaryLocation W20451641011 @default.
- W2045164101 hasRelatedWork W1591820460 @default.
- W2045164101 hasRelatedWork W161677908 @default.
- W2045164101 hasRelatedWork W2013059171 @default.
- W2045164101 hasRelatedWork W2045164101 @default.
- W2045164101 hasRelatedWork W2063836141 @default.
- W2045164101 hasRelatedWork W2072182670 @default.
- W2045164101 hasRelatedWork W2118672303 @default.
- W2045164101 hasRelatedWork W2159284166 @default.
- W2045164101 hasRelatedWork W2365490899 @default.
- W2045164101 hasRelatedWork W4247780997 @default.
- W2045164101 hasVolume "31" @default.
- W2045164101 isParatext "false" @default.
- W2045164101 isRetracted "false" @default.
- W2045164101 magId "2045164101" @default.
- W2045164101 workType "article" @default.