FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045189668> ?p ?o ?g. }

• W2045189668 endingPage "371" @default.
• W2045189668 startingPage "366" @default.
• W2045189668 abstract "We developed a novel xylose-utilizing ethanologenic Escherichia coli strain FBR3 with the potential for fermentation of mixed sugars from lignocellulosic hydrolysates into ethanol in an antibiotic-free media. This stain carries the plasmid pLOI297 which contains the genes from Zymomonas mobilis necessary for efficiently converting pyruvate into ethanol. Strain FBR3 selectively maintains pLOI297 when grown anaerobically. Cultures of strain FBR3 were serially transferred ten times in aerobic and anaerobic cultures containing either glucose or xylose with no selective antibiotic. An average of 97.4 ± 3.5% of the cells maintained pLOI297 in anaerobic cultures. In contrast, the plasmid quickly disappeared from aerobic cultures. Plasmid maintenance depends upon deletion of two enzyme activities: pyruvate formate lyase (pfl) and lactate dehydrogenase (ldh). The stability of the pfl mutation was confirmed by the absence of hydrogen gas production, an indirect assay for pfl activity, in each of the cultures. The FBR3 strain was transferred on xylose-containing medium and tested in pH-controlled batch fermentations for efficient conversion of pentoses and hexoses into ethanol. The batch medium contained either 10% (w/v) arabinose, glucose, xylose, or a mixture of these sugars. Fermentations were completed in 70–80 h and ethanol yields were 90–91% of theoretical; maximum ethanol concentrations were 4.38–4.66% (w/v). The fermentation performance of the new FBR3 strain compared favorably to the previously reported performance of strain K011." @default.
• W2045189668 created "2016-06-24" @default.
• W2045189668 creator A5002036292 @default.
• W2045189668 creator A5002311653 @default.
• W2045189668 creator A5042163043 @default.
• W2045189668 creator A5045790330 @default.
• W2045189668 date "1998-11-01" @default.
• W2045189668 modified "2023-10-17" @default.
• W2045189668 title "Fermentation of hexose and pentose sugars using a novel ethanologenic Escherichia coli strain" @default.
• W2045189668 cites W1496345318 @default.
• W2045189668 cites W1583901171 @default.
• W2045189668 cites W1590308370 @default.
• W2045189668 cites W1655837444 @default.
• W2045189668 cites W1786840062 @default.
• W2045189668 cites W2030456411 @default.
• W2045189668 cites W2033932941 @default.
• W2045189668 cites W2046488634 @default.
• W2045189668 cites W2053906763 @default.
• W2045189668 cites W2058364495 @default.
• W2045189668 cites W2072664545 @default.
• W2045189668 cites W2085117677 @default.
• W2045189668 cites W2090678681 @default.
• W2045189668 cites W2127305119 @default.
• W2045189668 cites W2134011061 @default.
• W2045189668 cites W2154402996 @default.
• W2045189668 cites W2218155072 @default.
• W2045189668 cites W4242082306 @default.
• W2045189668 cites W4245220017 @default.
• W2045189668 cites W4252539754 @default.
• W2045189668 doi "https://doi.org/10.1016/s0141-0229(98)00064-7" @default.
• W2045189668 hasPublicationYear "1998" @default.
• W2045189668 type Work @default.
• W2045189668 sameAs 2045189668 @default.
• W2045189668 citedByCount "59" @default.
• W2045189668 countsByYear W20451896682012 @default.
• W2045189668 countsByYear W20451896682013 @default.
• W2045189668 countsByYear W20451896682014 @default.
• W2045189668 countsByYear W20451896682015 @default.
• W2045189668 countsByYear W20451896682016 @default.
• W2045189668 countsByYear W20451896682018 @default.
• W2045189668 countsByYear W20451896682020 @default.
• W2045189668 countsByYear W20451896682022 @default.
• W2045189668 countsByYear W20451896682023 @default.
• W2045189668 crossrefType "journal-article" @default.
• W2045189668 hasAuthorship W2045189668A5002036292 @default.
• W2045189668 hasAuthorship W2045189668A5002311653 @default.
• W2045189668 hasAuthorship W2045189668A5042163043 @default.
• W2045189668 hasAuthorship W2045189668A5045790330 @default.
• W2045189668 hasConcept C100544194 @default.
• W2045189668 hasConcept C104317684 @default.
• W2045189668 hasConcept C105702510 @default.
• W2045189668 hasConcept C176221332 @default.
• W2045189668 hasConcept C181199279 @default.
• W2045189668 hasConcept C185592680 @default.
• W2045189668 hasConcept C20251656 @default.
• W2045189668 hasConcept C2775920511 @default.
• W2045189668 hasConcept C2776084176 @default.
• W2045189668 hasConcept C2776393687 @default.
• W2045189668 hasConcept C2777344606 @default.
• W2045189668 hasConcept C2778022156 @default.
• W2045189668 hasConcept C2780161600 @default.
• W2045189668 hasConcept C2780261296 @default.
• W2045189668 hasConcept C2780294150 @default.
• W2045189668 hasConcept C2780604136 @default.
• W2045189668 hasConcept C3409486 @default.
• W2045189668 hasConcept C523546767 @default.
• W2045189668 hasConcept C53850387 @default.
• W2045189668 hasConcept C54355233 @default.
• W2045189668 hasConcept C547475151 @default.
• W2045189668 hasConcept C55493867 @default.
• W2045189668 hasConcept C65183824 @default.
• W2045189668 hasConcept C86803240 @default.
• W2045189668 hasConcept C98861065 @default.
• W2045189668 hasConceptScore W2045189668C100544194 @default.
• W2045189668 hasConceptScore W2045189668C104317684 @default.
• W2045189668 hasConceptScore W2045189668C105702510 @default.
• W2045189668 hasConceptScore W2045189668C176221332 @default.
• W2045189668 hasConceptScore W2045189668C181199279 @default.
• W2045189668 hasConceptScore W2045189668C185592680 @default.
• W2045189668 hasConceptScore W2045189668C20251656 @default.
• W2045189668 hasConceptScore W2045189668C2775920511 @default.
• W2045189668 hasConceptScore W2045189668C2776084176 @default.
• W2045189668 hasConceptScore W2045189668C2776393687 @default.
• W2045189668 hasConceptScore W2045189668C2777344606 @default.
• W2045189668 hasConceptScore W2045189668C2778022156 @default.
• W2045189668 hasConceptScore W2045189668C2780161600 @default.
• W2045189668 hasConceptScore W2045189668C2780261296 @default.
• W2045189668 hasConceptScore W2045189668C2780294150 @default.
• W2045189668 hasConceptScore W2045189668C2780604136 @default.
• W2045189668 hasConceptScore W2045189668C3409486 @default.
• W2045189668 hasConceptScore W2045189668C523546767 @default.
• W2045189668 hasConceptScore W2045189668C53850387 @default.
• W2045189668 hasConceptScore W2045189668C54355233 @default.
• W2045189668 hasConceptScore W2045189668C547475151 @default.
• W2045189668 hasConceptScore W2045189668C55493867 @default.
• W2045189668 hasConceptScore W2045189668C65183824 @default.
• W2045189668 hasConceptScore W2045189668C86803240 @default.
• W2045189668 hasConceptScore W2045189668C98861065 @default.

• next