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• W2045204908 startingPage "e34063" @default.
• W2045204908 abstract "This paper describes the construction and characterization of a system of transcriptional reporter genes for monitoring the activity of signaling pathways and gene regulation mechanisms in intact Drosophila, dissected tissues or cultured cells. Transgenic integration of the reporters into the Drosophila germline was performed in a site-directed manner, using ΦC31 integrase. This strategy avoids variable position effects and assures low base level activity and high signal responsiveness. Defined integration sites furthermore enable the experimenter to compare the activity of different reporters in one organism. The reporter constructs have a modular design to facilitate the combination of promoter elements (synthetic transcription factor binding sites or natural regulatory sequences), reporter genes (eGFP, or DsRed.T4), and genomic integration sites. The system was used to analyze and compare the activity and signal response profiles of two stress inducible transcription factors, AP-1 and Nrf2. To complement the transgenic reporter fly lines, tissue culture assays were developed in which the same synthetic ARE and TRE elements control the expression of firefly luciferase." @default.
• W2045204908 created "2016-06-24" @default.
• W2045204908 creator A5036559527 @default.
• W2045204908 creator A5044917668 @default.
• W2045204908 date "2012-04-11" @default.
• W2045204908 modified "2023-10-16" @default.
• W2045204908 title "A Versatile ΦC31 Based Reporter System for Measuring AP-1 and Nrf2 Signaling in Drosophila and in Tissue Culture" @default.
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• W2045204908 doi "https://doi.org/10.1371/journal.pone.0034063" @default.
• W2045204908 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3324472" @default.
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