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• W2045218018 abstract "The pinewood nematode, Bursaphelenchus xylophilus, has caused significant damage to pine plantations both in East Asia and North America and is an important quarantine organism. A real-time polymerase chain reaction (PCR) assay was developed to detect B. xylophilus. A set of primers and probe specific for B. xylophilus was designed to target the ribosomal DNA internal transcribed spacer region. Optimal primer concentration, Mg 2+ concentration, and extension temperature were 400 nM, 3.0 mM, and 60°C, respectively. The assay was highly specific and sensitive, detecting as little as 0.01 ng of B. xylophilus DNA. The real-time PCR assay also successfully detected B. xylophilus in field samples, and it should be very useful for quarantine purposes." @default.
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• W2045218018 date "2005-05-01" @default.
• W2045218018 modified "2023-09-27" @default.
• W2045218018 title "Detection of the Pinewood Nematode, <i>Bursaphelenchus xylophilus</i>, Using a Real-Time Polymerase Chain Reaction Assay" @default.
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• W2045218018 doi "https://doi.org/10.1094/phyto-95-0566" @default.
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