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- W2045220911 abstract "Biodiesel is emerging as a major renewable energy resource. Glycerol is byproduct of biodiesel production, which cannot be directly used as fuel. Using <i>Pichia pastoris</i> as aerobic yeast cell factory, we engineered pathway to convert glycerol to propylene glycol (PG; 1,2-propanediol, 1,2-dihydroxypropane), a fuel additive and commodity compound. PG biosynthetic pathway was designed with unique combination of 3 enzymes: <i>Escherichia coli</i> methylglyoxal synthase and alcohol dehydrogenase; and <i>Pichia ofunaensis </i>glycerol dehydrogenase. Engineered genes were successfully integrated in yeast genome and actively expressed. Analysis, by HPLC of shake flask and fermenter broths of glycerol-grown cells, demonstrated engineered yeast produced PG. In fermenter, highest PG concentration was 0.11 g/L. When engineered <i>P. pastoris</i> was grown on biodiesel crude glycerol as sole carbon source, PG was detected. Basically, this is a proof of concept to demonstrate that <i>Pichia</i> was engineered to produce PG from glycerol." @default.
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- W2045220911 date "2012-08-31" @default.
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- W2045220911 title "Genetic Modification of Pichia Pastoris for Production of Propylene Glycol from Glycerol" @default.
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- W2045220911 doi "https://doi.org/10.5923/j.ijge.20110101.02" @default.
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