FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045233218> ?p ?o ?g. }

• W2045233218 endingPage "e0116726" @default.
• W2045233218 startingPage "e0116726" @default.
• W2045233218 abstract "Mitochondrial DNA/protein complexes (nucleoids) appear as discrete entities inside the mitochondrial network when observed by live-cell imaging and immunofluorescence. This somewhat trivial observation in recent years has spurred research towards isolation of these complexes and the identification of nucleoid-associated proteins. Here we show that whole cell formaldehyde crosslinking combined with affinity purification and tandem mass-spectrometry provides a simple and reproducible method to identify potential nucleoid associated proteins. The method avoids spurious mitochondrial isolation and subsequent multifarious nucleoid enrichment protocols and can be implemented to allow for label-free quantification (LFQ) by mass-spectrometry. Using expression of a Flag-tagged Twinkle helicase and appropriate controls we show that this method identifies many previously identified nucleoid associated proteins. Using LFQ to compare HEK293 cells with and without mtDNA, but both expressing Twinkle-FLAG, identifies many proteins that are reduced or absent in the absence of mtDNA. This set not only includes established mtDNA maintenance proteins but also many proteins involved in mitochondrial RNA metabolism and translation and therefore represents what can be considered an mtDNA gene expression proteome. Our data provides a very valuable resource for both basic mitochondrial researchers as well as clinical geneticists working to identify novel disease genes on the basis of exome sequence data." @default.
• W2045233218 created "2016-06-24" @default.
• W2045233218 creator A5006084516 @default.
• W2045233218 creator A5039974912 @default.
• W2045233218 creator A5043173438 @default.
• W2045233218 creator A5043797050 @default.
• W2045233218 creator A5061175424 @default.
• W2045233218 creator A5081336330 @default.
• W2045233218 date "2015-02-19" @default.
• W2045233218 modified "2023-10-16" @default.
• W2045233218 title "Whole Cell Formaldehyde Cross-Linking Simplifies Purification of Mitochondrial Nucleoids and Associated Proteins Involved in Mitochondrial Gene Expression" @default.
• W2045233218 cites W1515500852 @default.
• W2045233218 cites W1548557487 @default.
• W2045233218 cites W1551996591 @default.
• W2045233218 cites W1737281046 @default.
• W2045233218 cites W1801560648 @default.
• W2045233218 cites W1969239250 @default.
• W2045233218 cites W1977368092 @default.
• W2045233218 cites W1985990508 @default.
• W2045233218 cites W1986911150 @default.
• W2045233218 cites W1988888878 @default.
• W2045233218 cites W1992477424 @default.
• W2045233218 cites W1994093290 @default.
• W2045233218 cites W1996592585 @default.
• W2045233218 cites W1997496553 @default.
• W2045233218 cites W1999613693 @default.
• W2045233218 cites W2001756549 @default.
• W2045233218 cites W2003236418 @default.
• W2045233218 cites W2008038817 @default.
• W2045233218 cites W2010463336 @default.
• W2045233218 cites W2025779835 @default.
• W2045233218 cites W2025862351 @default.
• W2045233218 cites W2030368407 @default.
• W2045233218 cites W2032386922 @default.
• W2045233218 cites W2041440237 @default.
• W2045233218 cites W2041959633 @default.
• W2045233218 cites W2047293960 @default.
• W2045233218 cites W2048485231 @default.
• W2045233218 cites W2057289695 @default.
• W2045233218 cites W2059430355 @default.
• W2045233218 cites W2059453827 @default.
• W2045233218 cites W2075195786 @default.
• W2045233218 cites W2076358214 @default.
• W2045233218 cites W2080752012 @default.
• W2045233218 cites W2081678667 @default.
• W2045233218 cites W2084760480 @default.
• W2045233218 cites W2084900854 @default.
• W2045233218 cites W2087434357 @default.
• W2045233218 cites W2089172085 @default.
• W2045233218 cites W2089717004 @default.
• W2045233218 cites W2091701724 @default.
• W2045233218 cites W2091937165 @default.
• W2045233218 cites W2105435807 @default.
• W2045233218 cites W2106099798 @default.
• W2045233218 cites W2116459974 @default.
• W2045233218 cites W2119165025 @default.
• W2045233218 cites W2122914905 @default.
• W2045233218 cites W2126194787 @default.
• W2045233218 cites W2128417824 @default.
• W2045233218 cites W2132130208 @default.
• W2045233218 cites W2144082713 @default.
• W2045233218 cites W2154068452 @default.
• W2045233218 cites W2158198678 @default.
• W2045233218 cites W2159447177 @default.
• W2045233218 cites W2160824763 @default.
• W2045233218 cites W2165669681 @default.
• W2045233218 cites W2165988320 @default.
• W2045233218 cites W2168754435 @default.
• W2045233218 cites W2171666431 @default.
• W2045233218 doi "https://doi.org/10.1371/journal.pone.0116726" @default.
• W2045233218 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4335056" @default.
• W2045233218 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25695250" @default.
• W2045233218 hasPublicationYear "2015" @default.
• W2045233218 type Work @default.
• W2045233218 sameAs 2045233218 @default.
• W2045233218 citedByCount "31" @default.
• W2045233218 countsByYear W20452332182015 @default.
• W2045233218 countsByYear W20452332182016 @default.
• W2045233218 countsByYear W20452332182017 @default.
• W2045233218 countsByYear W20452332182018 @default.
• W2045233218 countsByYear W20452332182019 @default.
• W2045233218 countsByYear W20452332182020 @default.
• W2045233218 countsByYear W20452332182021 @default.
• W2045233218 countsByYear W20452332182022 @default.
• W2045233218 countsByYear W20452332182023 @default.
• W2045233218 crossrefType "journal-article" @default.
• W2045233218 hasAuthorship W2045233218A5006084516 @default.
• W2045233218 hasAuthorship W2045233218A5039974912 @default.
• W2045233218 hasAuthorship W2045233218A5043173438 @default.
• W2045233218 hasAuthorship W2045233218A5043797050 @default.
• W2045233218 hasAuthorship W2045233218A5061175424 @default.
• W2045233218 hasAuthorship W2045233218A5081336330 @default.
• W2045233218 hasBestOaLocation W20452332181 @default.
• W2045233218 hasConcept C104317684 @default.
• W2045233218 hasConcept C104397665 @default.
• W2045233218 hasConcept C150194340 @default.
• W2045233218 hasConcept C153911025 @default.
• W2045233218 hasConcept C162317418 @default.

• next