Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045235527> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2045235527 endingPage "397" @default.
- W2045235527 startingPage "393" @default.
- W2045235527 abstract "A method is proposed for the determination of the dissociation constants of enzyme-cofactor complexes which depends on the alteration, by the cofactor, of the rate of inhibition of the enzyme by a slow-acting, irreversible inhibitor. The method is tested with pyruvate kinase (EC 2.7.1.40) which is inhibited by p-mercuribenzoate in a slow second-order reaction (k = (3.8 ± 0.8)·103 mole−1sec−1) and is protected by the co-factor Mn2+. The dissociation constant of the manganese-pyruvate kinase complex determined by this method (Kd = (0.47 ± 0.16)·10−4 M) at 5·10−9 M enzyme is in good agreement with the activator constant of Mn2+, and with Kd obtained by three independent types of direct binding studies (electron-spin resonance, proton-relaxation rate, and ultraviolet difference spectroscopy) carried out at (3–10)·10−5 M enzyme. The major advantage of the present method is that it permits the determination of Kd at extremely low concentrations of enzymes. Other advantages and disadvantages of this method are enumerated." @default.
- W2045235527 created "2016-06-24" @default.
- W2045235527 creator A5071455712 @default.
- W2045235527 creator A5086742789 @default.
- W2045235527 date "1964-09-01" @default.
- W2045235527 modified "2023-09-26" @default.
- W2045235527 title "Determination of co-factor dissociation constants from the kinetics of inhibition of enzymes" @default.
- W2045235527 cites W1559218659 @default.
- W2045235527 cites W1637750471 @default.
- W2045235527 cites W176226702 @default.
- W2045235527 cites W1974793512 @default.
- W2045235527 cites W2048418049 @default.
- W2045235527 cites W2092637774 @default.
- W2045235527 cites W2333363395 @default.
- W2045235527 doi "https://doi.org/10.1016/0926-6569(64)90065-3" @default.
- W2045235527 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14209322" @default.
- W2045235527 hasPublicationYear "1964" @default.
- W2045235527 type Work @default.
- W2045235527 sameAs 2045235527 @default.
- W2045235527 citedByCount "20" @default.
- W2045235527 crossrefType "journal-article" @default.
- W2045235527 hasAuthorship W2045235527A5071455712 @default.
- W2045235527 hasAuthorship W2045235527A5086742789 @default.
- W2045235527 hasConcept C102931765 @default.
- W2045235527 hasConcept C121332964 @default.
- W2045235527 hasConcept C147789679 @default.
- W2045235527 hasConcept C148898269 @default.
- W2045235527 hasConcept C170493617 @default.
- W2045235527 hasConcept C181199279 @default.
- W2045235527 hasConcept C185592680 @default.
- W2045235527 hasConcept C197957613 @default.
- W2045235527 hasConcept C41183919 @default.
- W2045235527 hasConcept C55493867 @default.
- W2045235527 hasConcept C56856141 @default.
- W2045235527 hasConcept C62520636 @default.
- W2045235527 hasConcept C71240020 @default.
- W2045235527 hasConcept C74998103 @default.
- W2045235527 hasConcept C93391505 @default.
- W2045235527 hasConceptScore W2045235527C102931765 @default.
- W2045235527 hasConceptScore W2045235527C121332964 @default.
- W2045235527 hasConceptScore W2045235527C147789679 @default.
- W2045235527 hasConceptScore W2045235527C148898269 @default.
- W2045235527 hasConceptScore W2045235527C170493617 @default.
- W2045235527 hasConceptScore W2045235527C181199279 @default.
- W2045235527 hasConceptScore W2045235527C185592680 @default.
- W2045235527 hasConceptScore W2045235527C197957613 @default.
- W2045235527 hasConceptScore W2045235527C41183919 @default.
- W2045235527 hasConceptScore W2045235527C55493867 @default.
- W2045235527 hasConceptScore W2045235527C56856141 @default.
- W2045235527 hasConceptScore W2045235527C62520636 @default.
- W2045235527 hasConceptScore W2045235527C71240020 @default.
- W2045235527 hasConceptScore W2045235527C74998103 @default.
- W2045235527 hasConceptScore W2045235527C93391505 @default.
- W2045235527 hasIssue "3" @default.
- W2045235527 hasLocation W20452355271 @default.
- W2045235527 hasLocation W20452355272 @default.
- W2045235527 hasOpenAccess W2045235527 @default.
- W2045235527 hasPrimaryLocation W20452355271 @default.
- W2045235527 hasRelatedWork W1869038516 @default.
- W2045235527 hasRelatedWork W1902860727 @default.
- W2045235527 hasRelatedWork W1974981147 @default.
- W2045235527 hasRelatedWork W1976580976 @default.
- W2045235527 hasRelatedWork W2031130901 @default.
- W2045235527 hasRelatedWork W2036663608 @default.
- W2045235527 hasRelatedWork W2091165889 @default.
- W2045235527 hasRelatedWork W2094905459 @default.
- W2045235527 hasRelatedWork W2321875965 @default.
- W2045235527 hasRelatedWork W4242836331 @default.
- W2045235527 hasVolume "89" @default.
- W2045235527 isParatext "false" @default.
- W2045235527 isRetracted "false" @default.
- W2045235527 magId "2045235527" @default.
- W2045235527 workType "article" @default.