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- W2045262870 abstract "Background&Aims: Maintenance of intracellular pH is crucial to normal cell function, as many cellular processes have a narrow pH optimum. Tumor cells possess high-glycolytic activity and produce acidic metabolites. Moreover, tumor cells often exist in a hypoxic microenvironment with a lower extracellular pH than that of surrounding normal cells. To survive in this harsh environment, tumor cells must exhibit a dynamic cytosolic pH regulatory system. In this study, we made an investigation on expressions of H+-regulated channels/ exchangers in native human hepatic carcinoma. Methods: The mRNA expressions of Na+/ H+ exchanger (NHE), H+-K+ ATPase, and vacuolar H+-ATPase (V-ATPase) subunit c were examined by real time PCR. The protein expressions were analyzed by Western Blot. The protein expression and intracellular distribution of V-ATPase subunit c was further analyzed by immunohistochemistry. Results: The expressions of mRNA and protein of H+-K+ ATPase were not detected in native human hepatic carcinoma and normal hepatic tissues. The mRNA and protein expression levels of NHE1 and NHE3 were similar in native human hepatic carcinoma and normal hepatic tissues. However, the mRNA and protein expression levels of V-ATPase subunit c in native human hepatic carcinoma were markedly increased, compared with normal hepatic tissues. Immunohistochemistry analysis further confirmed the enhanced expression of V-ATPase subunit c on the plasma membrane of hepatic carcinoma cells. Conclusion: The results demonstrated that the expression of V-ATPase in human hepatic carcinoma is markedly increased, indicating V-ATPase is possibly involved in the regulation of intracellular pH of hepatic carcinoma cells. V-ATPase may represent a potential target for a novel pharmacotherapy of hepatic carcinoma." @default.
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- W2045262870 date "2010-05-01" @default.
- W2045262870 modified "2023-09-29" @default.
- W2045262870 title "S1286 Enhanced Expression of Vacuolar H+-ATPase in Native Human Hepatic Carcinoma" @default.
- W2045262870 doi "https://doi.org/10.1016/s0016-5085(10)61002-5" @default.
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