Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045287850> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2045287850 endingPage "137" @default.
- W2045287850 startingPage "129" @default.
- W2045287850 abstract "The effects that haemoglobin–metabolite interactions have on estimates of free magnesium-ion concentration in human erythrocytes, determined by 31P NMR [Gupta, R. K. et al., J. Biol. Chem. 253, 6172–6176 (1978)], were investigated. If the metabolite–haemoglobin association constants of Berger et al. [Eur. J. Biochem. 38, 553–562 (1973)] are used in the analysis then the estimates of intracellular free magnesium-ion concentration made by Gupta et al. (0.25 and 0.67 mM) become 0.43 and 0.60 mM, for oxygenated and deoxygenated cells, respectively. In oxygenated cells, this difference is primarily due to the lower value of KHbMgATP, given by Berger et al. These newly calculated concentrations are in closer agreement with those of Flatman (0.40 mM for oxygenated cells; 0.62 mM for deoxygenated cells) [Flatman, P. W., J. Physiol. 300, 19–30 (1980)] obtained with the zero-point titration' method. In addition, the assumptions that the chemical shift separations between the α- and β-phosphorus resonances of ATP and MgATP are unchanged on association with Hb were shown to be false. Under normal intracellular conditions this may lead to errors of 5–10%. Much larger errors would be possible in cases where significant amounts of ATP or MgATP are bound to Hb. These outcomes place doubt on measurements of intracellular free Mg2+ concentration made using 31P NMR if there is no consideration given to the total concentration of 2,3-bisphosphoglycerate (BPG), ATP and Hb in the sample; the same principle would apply to other cell-types. © 1997 John Wiley & Sons, Ltd." @default.
- W2045287850 created "2016-06-24" @default.
- W2045287850 creator A5065920375 @default.
- W2045287850 creator A5081563103 @default.
- W2045287850 date "1997-05-01" @default.
- W2045287850 modified "2023-09-26" @default.
- W2045287850 title "Free magnesium-ion concentration in erythrocytes by31P NMR: the effect of metabolite–haemoglobin interactions" @default.
- W2045287850 cites W1191012606 @default.
- W2045287850 cites W1538274312 @default.
- W2045287850 cites W1581909905 @default.
- W2045287850 cites W1643161846 @default.
- W2045287850 cites W182032073 @default.
- W2045287850 cites W1963574488 @default.
- W2045287850 cites W1972432635 @default.
- W2045287850 cites W1976506304 @default.
- W2045287850 cites W1982894487 @default.
- W2045287850 cites W2006469650 @default.
- W2045287850 cites W2007121116 @default.
- W2045287850 cites W2007758696 @default.
- W2045287850 cites W2008874204 @default.
- W2045287850 cites W2014666095 @default.
- W2045287850 cites W2025207380 @default.
- W2045287850 cites W2027420838 @default.
- W2045287850 cites W2039162996 @default.
- W2045287850 cites W2047268837 @default.
- W2045287850 cites W2066872657 @default.
- W2045287850 cites W2083337102 @default.
- W2045287850 cites W2084210553 @default.
- W2045287850 cites W2092366763 @default.
- W2045287850 cites W2117025288 @default.
- W2045287850 cites W2122957111 @default.
- W2045287850 cites W2126306374 @default.
- W2045287850 cites W2155408996 @default.
- W2045287850 cites W2161078065 @default.
- W2045287850 cites W2397882972 @default.
- W2045287850 cites W2398910721 @default.
- W2045287850 cites W2401961963 @default.
- W2045287850 cites W2419179559 @default.
- W2045287850 cites W2469061851 @default.
- W2045287850 cites W4299591657 @default.
- W2045287850 cites W94967005 @default.
- W2045287850 doi "https://doi.org/10.1002/(sici)1099-1492(199705)10:3<129::aid-nbm459>3.0.co;2-2" @default.
- W2045287850 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9408922" @default.
- W2045287850 hasPublicationYear "1997" @default.
- W2045287850 type Work @default.
- W2045287850 sameAs 2045287850 @default.
- W2045287850 citedByCount "12" @default.
- W2045287850 countsByYear W20452878502012 @default.
- W2045287850 countsByYear W20452878502022 @default.
- W2045287850 crossrefType "journal-article" @default.
- W2045287850 hasAuthorship W2045287850A5065920375 @default.
- W2045287850 hasAuthorship W2045287850A5081563103 @default.
- W2045287850 hasConcept C113196181 @default.
- W2045287850 hasConcept C12554922 @default.
- W2045287850 hasConcept C145148216 @default.
- W2045287850 hasConcept C178790620 @default.
- W2045287850 hasConcept C185592680 @default.
- W2045287850 hasConcept C2777477808 @default.
- W2045287850 hasConcept C43617362 @default.
- W2045287850 hasConcept C543218039 @default.
- W2045287850 hasConcept C55493867 @default.
- W2045287850 hasConcept C79879829 @default.
- W2045287850 hasConcept C86803240 @default.
- W2045287850 hasConceptScore W2045287850C113196181 @default.
- W2045287850 hasConceptScore W2045287850C12554922 @default.
- W2045287850 hasConceptScore W2045287850C145148216 @default.
- W2045287850 hasConceptScore W2045287850C178790620 @default.
- W2045287850 hasConceptScore W2045287850C185592680 @default.
- W2045287850 hasConceptScore W2045287850C2777477808 @default.
- W2045287850 hasConceptScore W2045287850C43617362 @default.
- W2045287850 hasConceptScore W2045287850C543218039 @default.
- W2045287850 hasConceptScore W2045287850C55493867 @default.
- W2045287850 hasConceptScore W2045287850C79879829 @default.
- W2045287850 hasConceptScore W2045287850C86803240 @default.
- W2045287850 hasIssue "3" @default.
- W2045287850 hasLocation W20452878501 @default.
- W2045287850 hasLocation W20452878502 @default.
- W2045287850 hasOpenAccess W2045287850 @default.
- W2045287850 hasPrimaryLocation W20452878501 @default.
- W2045287850 hasRelatedWork W1825144172 @default.
- W2045287850 hasRelatedWork W2029576269 @default.
- W2045287850 hasRelatedWork W2052493351 @default.
- W2045287850 hasRelatedWork W2081537774 @default.
- W2045287850 hasRelatedWork W2086557392 @default.
- W2045287850 hasRelatedWork W2091346150 @default.
- W2045287850 hasRelatedWork W2137448504 @default.
- W2045287850 hasRelatedWork W2253284724 @default.
- W2045287850 hasRelatedWork W2358263088 @default.
- W2045287850 hasRelatedWork W311435467 @default.
- W2045287850 hasVolume "10" @default.
- W2045287850 isParatext "false" @default.
- W2045287850 isRetracted "false" @default.
- W2045287850 magId "2045287850" @default.
- W2045287850 workType "article" @default.