Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045289697> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2045289697 abstract "Small intestinal villi are projective microstructures from the mucosa that provide a large surface area for digestion and absorption. On the mucosa, intestinal epithelial cells undergo terminal differentiation in space - along the crypt-villus axis - until they slough off into the lumen. Despite this unique physiological feature, to date in vitro cultivation of the intestinal epithelial cells is routinely done at the planar tissue-culture surface. In this research, we fabricated a projective, 3-dimensional (3-D) tissue-culture environment to provide a physiologically relevant condition for establishing the enterocyte cell culture in vitro. We used the mouse small intestinal epithelium as the model and applied a microfabrication process, UV-LIGA, to generate an array of microneedles with a similar projective structure and size (height: 400μm, base: 135μm in diameter) as those of the duodenal villi. In addition, we shaped the LIGA-derived poly (lactic acid) microneedles by acetone/ethanol erosion to create a smooth tip structure for the engraftment of human Caco-2 enterocytes. The engineered villus array had a total surface area of 4.81 cm <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>2</sup> per sq.cm of planar surface, which led to a 2.48-fold increase in the cell number of enterocytes on the 3-D construct relative to that on the planar control surface. Our work presents an initial step toward constituting a physiological gut in vitro by using an engineering approach for large-scale preparation of the biomimetic small intestine." @default.
- W2045289697 created "2016-06-24" @default.
- W2045289697 creator A5032620535 @default.
- W2045289697 creator A5053927396 @default.
- W2045289697 creator A5054818585 @default.
- W2045289697 creator A5065200821 @default.
- W2045289697 creator A5083859974 @default.
- W2045289697 creator A5087933927 @default.
- W2045289697 date "2012-03-01" @default.
- W2045289697 modified "2023-10-16" @default.
- W2045289697 title "Engineering a biomimetic villus array for in vitro 3-dimensional culture of intestinal epithelial cells" @default.
- W2045289697 cites W1974215447 @default.
- W2045289697 cites W1985665104 @default.
- W2045289697 cites W1987151016 @default.
- W2045289697 cites W1994470556 @default.
- W2045289697 cites W2062528228 @default.
- W2045289697 cites W2074545908 @default.
- W2045289697 cites W2082647909 @default.
- W2045289697 cites W2083117071 @default.
- W2045289697 cites W2111465078 @default.
- W2045289697 cites W2127524388 @default.
- W2045289697 cites W2136342882 @default.
- W2045289697 cites W2155933813 @default.
- W2045289697 cites W2159155233 @default.
- W2045289697 cites W2164358915 @default.
- W2045289697 doi "https://doi.org/10.1109/nems.2012.6196763" @default.
- W2045289697 hasPublicationYear "2012" @default.
- W2045289697 type Work @default.
- W2045289697 sameAs 2045289697 @default.
- W2045289697 citedByCount "1" @default.
- W2045289697 countsByYear W20452896972013 @default.
- W2045289697 crossrefType "proceedings-article" @default.
- W2045289697 hasAuthorship W2045289697A5032620535 @default.
- W2045289697 hasAuthorship W2045289697A5053927396 @default.
- W2045289697 hasAuthorship W2045289697A5054818585 @default.
- W2045289697 hasAuthorship W2045289697A5065200821 @default.
- W2045289697 hasAuthorship W2045289697A5083859974 @default.
- W2045289697 hasAuthorship W2045289697A5087933927 @default.
- W2045289697 hasConcept C12554922 @default.
- W2045289697 hasConcept C134018914 @default.
- W2045289697 hasConcept C136229726 @default.
- W2045289697 hasConcept C153911025 @default.
- W2045289697 hasConcept C185592680 @default.
- W2045289697 hasConcept C192562407 @default.
- W2045289697 hasConcept C202751555 @default.
- W2045289697 hasConcept C2777882243 @default.
- W2045289697 hasConcept C2779604457 @default.
- W2045289697 hasConcept C49892992 @default.
- W2045289697 hasConcept C529295009 @default.
- W2045289697 hasConcept C54355233 @default.
- W2045289697 hasConcept C55493867 @default.
- W2045289697 hasConcept C66090201 @default.
- W2045289697 hasConcept C71924100 @default.
- W2045289697 hasConcept C81885089 @default.
- W2045289697 hasConcept C86803240 @default.
- W2045289697 hasConcept C95444343 @default.
- W2045289697 hasConceptScore W2045289697C12554922 @default.
- W2045289697 hasConceptScore W2045289697C134018914 @default.
- W2045289697 hasConceptScore W2045289697C136229726 @default.
- W2045289697 hasConceptScore W2045289697C153911025 @default.
- W2045289697 hasConceptScore W2045289697C185592680 @default.
- W2045289697 hasConceptScore W2045289697C192562407 @default.
- W2045289697 hasConceptScore W2045289697C202751555 @default.
- W2045289697 hasConceptScore W2045289697C2777882243 @default.
- W2045289697 hasConceptScore W2045289697C2779604457 @default.
- W2045289697 hasConceptScore W2045289697C49892992 @default.
- W2045289697 hasConceptScore W2045289697C529295009 @default.
- W2045289697 hasConceptScore W2045289697C54355233 @default.
- W2045289697 hasConceptScore W2045289697C55493867 @default.
- W2045289697 hasConceptScore W2045289697C66090201 @default.
- W2045289697 hasConceptScore W2045289697C71924100 @default.
- W2045289697 hasConceptScore W2045289697C81885089 @default.
- W2045289697 hasConceptScore W2045289697C86803240 @default.
- W2045289697 hasConceptScore W2045289697C95444343 @default.
- W2045289697 hasLocation W20452896971 @default.
- W2045289697 hasOpenAccess W2045289697 @default.
- W2045289697 hasPrimaryLocation W20452896971 @default.
- W2045289697 hasRelatedWork W109523419 @default.
- W2045289697 hasRelatedWork W142262936 @default.
- W2045289697 hasRelatedWork W1989725422 @default.
- W2045289697 hasRelatedWork W2014552276 @default.
- W2045289697 hasRelatedWork W2116997602 @default.
- W2045289697 hasRelatedWork W2270816418 @default.
- W2045289697 hasRelatedWork W2331437648 @default.
- W2045289697 hasRelatedWork W3142826365 @default.
- W2045289697 hasRelatedWork W323520099 @default.
- W2045289697 hasRelatedWork W2496107749 @default.
- W2045289697 isParatext "false" @default.
- W2045289697 isRetracted "false" @default.
- W2045289697 magId "2045289697" @default.
- W2045289697 workType "article" @default.