FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045310480> ?p ?o ?g. }

• W2045310480 endingPage "653" @default.
• W2045310480 startingPage "643" @default.
• W2045310480 abstract "Primary mouse keratinocytes in culture are induced to terminally differentiate by increasing extracellular Ca2+ concentrations (CaO) from 0.05 mM to ≥ 0.1 mM. The addition of Sr2+ (≥ 2.5 mM) to medium containing 0.05 mM Ca2+ induces focal stratification and terminal differentiation, which are similar to that found after increasing the CaO to 0.12 mM. Sr2+ in 0.05 mM Ca2+ medium induces the expression of the differentiation-specific keratins, keratin 1 (K1), keratin 10 (K10), and the granular cell marker, filaggrin, as determined by both immunoblotting and immunofluorescence. Sr2+ induces the expression of those differentiation markers in a dose dependent manner, with an optimal concentration of 5 mM. In the absence of Ca2+ in the medium, the Sr2+ effects are reduced, and Sr2+ is ineffective when both Ca2+ and serum are deleted from the medium. Sr2+ treatment increases the ratio of fluorescence intensity of the intracellular Ca2+ sensitive probe, fura-2, indicating an associated rise in the level of intracellular free Ca2+ and/or Sr2+. At doses sufficient to induce differentiation, Sr2+ also increases the level of inositol phosphates in primary keratinocytes within 30 min. The uptake curves of 85Sr2+ by primary keratinocytes are similar to those of 45Ca2+. At low concentrations, the initial uptake of both 45Ca2+ and 85Sr2+ reaches a plateau within 1 hr; at higher concentrations, the uptake of both 45Ca2+ and 85Sr2+ increases continuously for 12 hr. In keratinocytes pre-equilibrated with 45Ca2+ in 0.05 mM Ca2+ medium, Sr2+ causes an increase of 45Ca2+ uptake, which is dependent on the presence of serum. These results suggest that Sr2+ utilizes the same signalling pathway as Ca2+ to induce keratinocyte terminal differentiation and that Ca2+ may be required to exert these effects. © 1993 Wiley-Liss, Inc." @default.
• W2045310480 created "2016-06-24" @default.
• W2045310480 creator A5018422229 @default.
• W2045310480 creator A5035360585 @default.
• W2045310480 creator A5045531616 @default.
• W2045310480 creator A5073998688 @default.
• W2045310480 creator A5080603462 @default.
• W2045310480 creator A5084047654 @default.
• W2045310480 date "1993-03-01" @default.
• W2045310480 modified "2023-10-16" @default.
• W2045310480 title "Strontium induces murine keratinocyte differentiation in vitro in the presence of serum and calcium" @default.
• W2045310480 cites W1486602990 @default.
• W2045310480 cites W1491383374 @default.
• W2045310480 cites W1571327651 @default.
• W2045310480 cites W1581807906 @default.
• W2045310480 cites W1585941552 @default.
• W2045310480 cites W1596708629 @default.
• W2045310480 cites W1599670156 @default.
• W2045310480 cites W1967840065 @default.
• W2045310480 cites W1981694430 @default.
• W2045310480 cites W1990433146 @default.
• W2045310480 cites W1991554549 @default.
• W2045310480 cites W1991856499 @default.
• W2045310480 cites W1993169352 @default.
• W2045310480 cites W1993212221 @default.
• W2045310480 cites W1997093700 @default.
• W2045310480 cites W1998464125 @default.
• W2045310480 cites W2001692359 @default.
• W2045310480 cites W2001716659 @default.
• W2045310480 cites W2010390522 @default.
• W2045310480 cites W2012960732 @default.
• W2045310480 cites W2016435958 @default.
• W2045310480 cites W2018267354 @default.
• W2045310480 cites W2026023235 @default.
• W2045310480 cites W2027250767 @default.
• W2045310480 cites W2031030609 @default.
• W2045310480 cites W2038085058 @default.
• W2045310480 cites W2054480906 @default.
• W2045310480 cites W2058887697 @default.
• W2045310480 cites W2061843934 @default.
• W2045310480 cites W2069771076 @default.
• W2045310480 cites W2073638852 @default.
• W2045310480 cites W2074646364 @default.
• W2045310480 cites W2082076255 @default.
• W2045310480 cites W2089101401 @default.
• W2045310480 cites W2100837269 @default.
• W2045310480 cites W2106142505 @default.
• W2045310480 cites W2111655918 @default.
• W2045310480 cites W2120243015 @default.
• W2045310480 cites W2132644765 @default.
• W2045310480 cites W2252017425 @default.
• W2045310480 cites W2479094108 @default.
• W2045310480 cites W268676397 @default.
• W2045310480 cites W58256895 @default.
• W2045310480 cites W656547784 @default.
• W2045310480 doi "https://doi.org/10.1002/jcp.1041540324" @default.
• W2045310480 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7679679" @default.
• W2045310480 hasPublicationYear "1993" @default.
• W2045310480 type Work @default.
• W2045310480 sameAs 2045310480 @default.
• W2045310480 citedByCount "18" @default.
• W2045310480 countsByYear W20453104802016 @default.
• W2045310480 crossrefType "journal-article" @default.
• W2045310480 hasAuthorship W2045310480A5018422229 @default.
• W2045310480 hasAuthorship W2045310480A5035360585 @default.
• W2045310480 hasAuthorship W2045310480A5045531616 @default.
• W2045310480 hasAuthorship W2045310480A5073998688 @default.
• W2045310480 hasAuthorship W2045310480A5080603462 @default.
• W2045310480 hasAuthorship W2045310480A5084047654 @default.
• W2045310480 hasConcept C12554922 @default.
• W2045310480 hasConcept C153911025 @default.
• W2045310480 hasConcept C159654299 @default.
• W2045310480 hasConcept C178790620 @default.
• W2045310480 hasConcept C185592680 @default.
• W2045310480 hasConcept C202751555 @default.
• W2045310480 hasConcept C203014093 @default.
• W2045310480 hasConcept C2777074287 @default.
• W2045310480 hasConcept C2778236600 @default.
• W2045310480 hasConcept C28406088 @default.
• W2045310480 hasConcept C519063684 @default.
• W2045310480 hasConcept C55493867 @default.
• W2045310480 hasConcept C74884574 @default.
• W2045310480 hasConcept C79879829 @default.
• W2045310480 hasConcept C86803240 @default.
• W2045310480 hasConcept C95444343 @default.
• W2045310480 hasConceptScore W2045310480C12554922 @default.
• W2045310480 hasConceptScore W2045310480C153911025 @default.
• W2045310480 hasConceptScore W2045310480C159654299 @default.
• W2045310480 hasConceptScore W2045310480C178790620 @default.
• W2045310480 hasConceptScore W2045310480C185592680 @default.
• W2045310480 hasConceptScore W2045310480C202751555 @default.
• W2045310480 hasConceptScore W2045310480C203014093 @default.
• W2045310480 hasConceptScore W2045310480C2777074287 @default.
• W2045310480 hasConceptScore W2045310480C2778236600 @default.
• W2045310480 hasConceptScore W2045310480C28406088 @default.
• W2045310480 hasConceptScore W2045310480C519063684 @default.
• W2045310480 hasConceptScore W2045310480C55493867 @default.
• W2045310480 hasConceptScore W2045310480C74884574 @default.

• next