Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045326496> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2045326496 endingPage "1219" @default.
- W2045326496 startingPage "1211" @default.
- W2045326496 abstract "To improve gene transfer activity of a new nonviral vector, a polyamidoamine dendrimer (G2) conjugate with alpha-cyclodextrin (alpha-CDE conjugate (G2)), we prepared alpha-CDE conjugates with dendrimer having different generations (G3 and G4), and their gene transfer activities were compared with those of alpha-CDE conjugate (G2) and TransFast, a novel transfection reagent. alpha-CDE conjugates (G2, G3, and G4) formed the complexes with pDNA, changing the zeta-potential and particle size of pDNA complexes and the protection of pDNA from DNase I in a charge ratio-dependent manner, although their differences at higher charge ratios (vector/pDNA) were small. The gene transfer activity of alpha-CDE conjugates (G2, G3, and G4) was higher than that of the corresponding dendrimer alone in NIH3T3 and RAW264.7 cells. Of these CDE conjugates, alpha-CDE conjugate (G3) had a superior gene transfer activity which was comparable to that of TransFast in NIH3T3 cells. The intracellular distribution of pDNA after application of the pDNA complex with alpha-CDE conjugate (G3) to NIH3T3 cells was different from that with dendrimer alone (G3), although the cellular association of pDNA was almost comparable among all vectors. alpha-CDE conjugate (G3) strongly interacted with a fluorescence probe, 2-(p-toluidinyl)-naphthalene-6-sulfonate (TNS), suggesting that the conjugate possesses the inclusion ability with biomembrane constituents such as phospholipids after transfection. These results suggest that alpha-CDE conjugates, particularly the G3 conjugate, could be novel nonviral gene transfer agents." @default.
- W2045326496 created "2016-06-24" @default.
- W2045326496 creator A5015362633 @default.
- W2045326496 creator A5017389314 @default.
- W2045326496 creator A5045294560 @default.
- W2045326496 creator A5059101174 @default.
- W2045326496 creator A5086855818 @default.
- W2045326496 date "2002-11-01" @default.
- W2045326496 modified "2023-10-17" @default.
- W2045326496 title "Effects of Structure of Polyamidoamine Dendrimer on Gene Transfer Efficiency of the Dendrimer Conjugate with α-Cyclodextrin" @default.
- W2045326496 cites W1972890442 @default.
- W2045326496 cites W1980414709 @default.
- W2045326496 cites W1988513927 @default.
- W2045326496 cites W1993863928 @default.
- W2045326496 cites W2043521465 @default.
- W2045326496 cites W2054159161 @default.
- W2045326496 cites W2055091943 @default.
- W2045326496 cites W2067882022 @default.
- W2045326496 cites W2074830928 @default.
- W2045326496 cites W2123237567 @default.
- W2045326496 cites W2154014672 @default.
- W2045326496 cites W2168206768 @default.
- W2045326496 cites W4233220338 @default.
- W2045326496 cites W4236921821 @default.
- W2045326496 cites W4240681501 @default.
- W2045326496 doi "https://doi.org/10.1021/bc025557d" @default.
- W2045326496 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12440855" @default.
- W2045326496 hasPublicationYear "2002" @default.
- W2045326496 type Work @default.
- W2045326496 sameAs 2045326496 @default.
- W2045326496 citedByCount "135" @default.
- W2045326496 countsByYear W20453264962012 @default.
- W2045326496 countsByYear W20453264962013 @default.
- W2045326496 countsByYear W20453264962014 @default.
- W2045326496 countsByYear W20453264962015 @default.
- W2045326496 countsByYear W20453264962016 @default.
- W2045326496 countsByYear W20453264962017 @default.
- W2045326496 countsByYear W20453264962018 @default.
- W2045326496 countsByYear W20453264962019 @default.
- W2045326496 countsByYear W20453264962020 @default.
- W2045326496 countsByYear W20453264962021 @default.
- W2045326496 countsByYear W20453264962022 @default.
- W2045326496 countsByYear W20453264962023 @default.
- W2045326496 crossrefType "journal-article" @default.
- W2045326496 hasAuthorship W2045326496A5015362633 @default.
- W2045326496 hasAuthorship W2045326496A5017389314 @default.
- W2045326496 hasAuthorship W2045326496A5045294560 @default.
- W2045326496 hasAuthorship W2045326496A5059101174 @default.
- W2045326496 hasAuthorship W2045326496A5086855818 @default.
- W2045326496 hasConcept C104317684 @default.
- W2045326496 hasConcept C12554922 @default.
- W2045326496 hasConcept C134306372 @default.
- W2045326496 hasConcept C135983454 @default.
- W2045326496 hasConcept C185592680 @default.
- W2045326496 hasConcept C197336794 @default.
- W2045326496 hasConcept C2779433975 @default.
- W2045326496 hasConcept C33923547 @default.
- W2045326496 hasConcept C54009773 @default.
- W2045326496 hasConcept C55493867 @default.
- W2045326496 hasConcept C55637122 @default.
- W2045326496 hasConcept C86803240 @default.
- W2045326496 hasConceptScore W2045326496C104317684 @default.
- W2045326496 hasConceptScore W2045326496C12554922 @default.
- W2045326496 hasConceptScore W2045326496C134306372 @default.
- W2045326496 hasConceptScore W2045326496C135983454 @default.
- W2045326496 hasConceptScore W2045326496C185592680 @default.
- W2045326496 hasConceptScore W2045326496C197336794 @default.
- W2045326496 hasConceptScore W2045326496C2779433975 @default.
- W2045326496 hasConceptScore W2045326496C33923547 @default.
- W2045326496 hasConceptScore W2045326496C54009773 @default.
- W2045326496 hasConceptScore W2045326496C55493867 @default.
- W2045326496 hasConceptScore W2045326496C55637122 @default.
- W2045326496 hasConceptScore W2045326496C86803240 @default.
- W2045326496 hasIssue "6" @default.
- W2045326496 hasLocation W20453264961 @default.
- W2045326496 hasLocation W20453264962 @default.
- W2045326496 hasOpenAccess W2045326496 @default.
- W2045326496 hasPrimaryLocation W20453264961 @default.
- W2045326496 hasRelatedWork W1567528935 @default.
- W2045326496 hasRelatedWork W2033029089 @default.
- W2045326496 hasRelatedWork W2052288565 @default.
- W2045326496 hasRelatedWork W2086182564 @default.
- W2045326496 hasRelatedWork W2313822410 @default.
- W2045326496 hasRelatedWork W2521389035 @default.
- W2045326496 hasRelatedWork W2523943737 @default.
- W2045326496 hasRelatedWork W2904616103 @default.
- W2045326496 hasRelatedWork W583401833 @default.
- W2045326496 hasRelatedWork W201818518 @default.
- W2045326496 hasVolume "13" @default.
- W2045326496 isParatext "false" @default.
- W2045326496 isRetracted "false" @default.
- W2045326496 magId "2045326496" @default.
- W2045326496 workType "article" @default.