FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045332651> ?p ?o ?g. }

• W2045332651 endingPage "15872" @default.
• W2045332651 startingPage "15863" @default.
• W2045332651 abstract "The binding characteristics of electropositive [LDL(+)] and electronegative LDL [LDL(−)] subfractions to the LDL receptor (LDLr) were studied. Saturation kinetic studies in cultured human fibroblasts demonstrated that LDL(−) from normolipemic (NL) and familial hypercholesterolemic (FH) subjects had lower binding affinity than their respective LDL(+) fractions (P < 0.05), as indicated by higher dissociation constant (KD) values. FH-LDL(+) also showed lower binding affinity (P < 0.05) than NL-LDL(+) (KD, sorted from lower to higher affinity: NL-LDL(−), 33.0 ± 24.4 nM; FH-LDL(−), 24.4 ± 7.1 nM; FH-LDL(+), 16.6 ± 7.0 nM; NL-LDL(+), 10.9 ± 5.7 nM). These results were confirmed by binding displacement studies. The impaired affinity binding of LDL(−) could be attributed to altered secondary and tertiary structure of apolipoprotein B, but circular dichroism (CD) and tryptophan fluorescence (TrpF) studies revealed no structural differences between LDL(+) and LDL(−). To ascertain the role of increased nonesterified fatty acids (NEFA) and lysophosphatidylcholine (LPC) content in LDL(−), LDL(+) was enriched in NEFA or hydrolyzed with secretory phospholipase A2. Modification of LDL gradually decreased the affinity to LDLr in parallel to the increasing content of NEFA and/or LPC. Modified LDLs with a NEFA content similar to that of LDL(−) displayed similar affinity. ApoB structure studies of modified LDLs by CD and TrpF showed no difference compared to LDL(+) or LDL(−). Our results indicate that NEFA loading or phospholipase A2 lipolysis of LDL leads to changes that affect the affinity of LDL to LDLr with no major effect on apoB structure. Impaired affinity to the LDLr shown by LDL(−) is related to NEFA and/or LPC content rather than to structural differences in apolipoprotein B." @default.
• W2045332651 created "2016-06-24" @default.
• W2045332651 creator A5015278177 @default.
• W2045332651 creator A5026456319 @default.
• W2045332651 creator A5037240373 @default.
• W2045332651 creator A5054081272 @default.
• W2045332651 creator A5056197299 @default.
• W2045332651 creator A5058557146 @default.
• W2045332651 creator A5076452941 @default.
• W2045332651 date "2004-11-24" @default.
• W2045332651 modified "2023-09-29" @default.
• W2045332651 title "Impaired Binding Affinity of Electronegative Low-Density Lipoprotein (LDL) to the LDL Receptor Is Related to Nonesterified Fatty Acids and Lysophosphatidylcholine Content" @default.
• W2045332651 cites W1533260020 @default.
• W2045332651 cites W1534702626 @default.
• W2045332651 cites W1869308072 @default.
• W2045332651 cites W1879201657 @default.
• W2045332651 cites W1937455559 @default.
• W2045332651 cites W1955961319 @default.
• W2045332651 cites W2022534250 @default.
• W2045332651 cites W2050776161 @default.
• W2045332651 cites W2056237978 @default.
• W2045332651 cites W2068619914 @default.
• W2045332651 cites W2081010186 @default.
• W2045332651 cites W2101356464 @default.
• W2045332651 cites W2115519661 @default.
• W2045332651 cites W2116717181 @default.
• W2045332651 cites W2162458714 @default.
• W2045332651 cites W2184987640 @default.
• W2045332651 cites W2188633998 @default.
• W2045332651 cites W2305216237 @default.
• W2045332651 cites W2313576785 @default.
• W2045332651 cites W2473455258 @default.
• W2045332651 doi "https://doi.org/10.1021/bi048825z" @default.
• W2045332651 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15595841" @default.
• W2045332651 hasPublicationYear "2004" @default.
• W2045332651 type Work @default.
• W2045332651 sameAs 2045332651 @default.
• W2045332651 citedByCount "46" @default.
• W2045332651 countsByYear W20453326512012 @default.
• W2045332651 countsByYear W20453326512013 @default.
• W2045332651 countsByYear W20453326512014 @default.
• W2045332651 countsByYear W20453326512015 @default.
• W2045332651 countsByYear W20453326512016 @default.
• W2045332651 countsByYear W20453326512017 @default.
• W2045332651 countsByYear W20453326512018 @default.
• W2045332651 countsByYear W20453326512019 @default.
• W2045332651 countsByYear W20453326512020 @default.
• W2045332651 countsByYear W20453326512021 @default.
• W2045332651 countsByYear W20453326512022 @default.
• W2045332651 countsByYear W20453326512023 @default.
• W2045332651 crossrefType "journal-article" @default.
• W2045332651 hasAuthorship W2045332651A5015278177 @default.
• W2045332651 hasAuthorship W2045332651A5026456319 @default.
• W2045332651 hasAuthorship W2045332651A5037240373 @default.
• W2045332651 hasAuthorship W2045332651A5054081272 @default.
• W2045332651 hasAuthorship W2045332651A5056197299 @default.
• W2045332651 hasAuthorship W2045332651A5058557146 @default.
• W2045332651 hasAuthorship W2045332651A5076452941 @default.
• W2045332651 hasConcept C126322002 @default.
• W2045332651 hasConcept C134018914 @default.
• W2045332651 hasConcept C170493617 @default.
• W2045332651 hasConcept C185592680 @default.
• W2045332651 hasConcept C2776330855 @default.
• W2045332651 hasConcept C2778163477 @default.
• W2045332651 hasConcept C2778596009 @default.
• W2045332651 hasConcept C2778918659 @default.
• W2045332651 hasConcept C2779620165 @default.
• W2045332651 hasConcept C2779772378 @default.
• W2045332651 hasConcept C2780072125 @default.
• W2045332651 hasConcept C41625074 @default.
• W2045332651 hasConcept C43554185 @default.
• W2045332651 hasConcept C543025807 @default.
• W2045332651 hasConcept C55493867 @default.
• W2045332651 hasConcept C62746215 @default.
• W2045332651 hasConcept C71924100 @default.
• W2045332651 hasConcept C74998103 @default.
• W2045332651 hasConcept C86803240 @default.
• W2045332651 hasConceptScore W2045332651C126322002 @default.
• W2045332651 hasConceptScore W2045332651C134018914 @default.
• W2045332651 hasConceptScore W2045332651C170493617 @default.
• W2045332651 hasConceptScore W2045332651C185592680 @default.
• W2045332651 hasConceptScore W2045332651C2776330855 @default.
• W2045332651 hasConceptScore W2045332651C2778163477 @default.
• W2045332651 hasConceptScore W2045332651C2778596009 @default.
• W2045332651 hasConceptScore W2045332651C2778918659 @default.
• W2045332651 hasConceptScore W2045332651C2779620165 @default.
• W2045332651 hasConceptScore W2045332651C2779772378 @default.
• W2045332651 hasConceptScore W2045332651C2780072125 @default.
• W2045332651 hasConceptScore W2045332651C41625074 @default.
• W2045332651 hasConceptScore W2045332651C43554185 @default.
• W2045332651 hasConceptScore W2045332651C543025807 @default.
• W2045332651 hasConceptScore W2045332651C55493867 @default.
• W2045332651 hasConceptScore W2045332651C62746215 @default.
• W2045332651 hasConceptScore W2045332651C71924100 @default.
• W2045332651 hasConceptScore W2045332651C74998103 @default.
• W2045332651 hasConceptScore W2045332651C86803240 @default.
• W2045332651 hasIssue "50" @default.
• W2045332651 hasLocation W20453326511 @default.

• next