Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045335093> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2045335093 endingPage "2643" @default.
- W2045335093 startingPage "2637" @default.
- W2045335093 abstract "To determine if both the Ca2+-calmodulin system and the cyclic AMP system may regulate tyrosine hydroxylase (TH) activity in situ, rat striatal tissue slices that contain all of the components of the TH, cyclic AMP and Ca2+-calmodulin systems were subjected to experimental manipulations. Incubation of striatal tissue slices in a medium containing W-7 [N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide], a specific inhibitor of calmodulin, resulted in a dose-dependent decrease of DOPA formation. The concentration of W-7 producing 50% inhibition (3 × 10−5 M) of DOPA formation was in good agreement with the binding affinity of W-7 to calmodulin. W-7 did not affect TH activity in vitro or cellular cyclic AMP level. A structurally unrelated calmodulin antagonist, trifluoroperazine, also inhibited DOPA formation. On the other hand, incubation of striatal tissue slices in a medium containing dibutyryl cyclic AMP (DBcAMP) increased DOPA formation dose dependently. Kinetic analysis revealed that the enzyme in homogenates of control tissue slices had two different Km values for a cofactor, 6-methyl-5,6,7,8-tetrahydropterin (6MPH4), indicating the presence of two forms in striatal tissue slices: a less active form with a relatively low affinity for the pterin cofactor and a more active form with a relatively high affinity. W-7 produced an increase of the high Km form and a decrease of the low Km form respectively. In contrast, incubation of tissue slices in the presence of DBcAMP resulted in almost complete activation of the enzyme to the low Km form. These results suggest that both the Ca2+-calmodulin-dependent system and the cyclic AMP-dependent system may regulate TH activity in the rat striatum in vivo. The different types of kinetic change produced by DBcAMP and W-7 indicate that the two processes may act in different fashions and that the basal level of catechol formation in striatal tissue slices may be dependent upon an activated form of TH." @default.
- W2045335093 created "2016-06-24" @default.
- W2045335093 creator A5004939550 @default.
- W2045335093 creator A5062895847 @default.
- W2045335093 date "1985-08-01" @default.
- W2045335093 modified "2023-09-24" @default.
- W2045335093 title "Evidence for the involvement of Ca2+-calmodulin and cyclic AMP in the regulation of the tyrosine hydroxylase system in rat striatal tissue slices" @default.
- W2045335093 cites W1497427077 @default.
- W2045335093 cites W1522783020 @default.
- W2045335093 cites W1598690553 @default.
- W2045335093 cites W1967518674 @default.
- W2045335093 cites W1974076630 @default.
- W2045335093 cites W2004810229 @default.
- W2045335093 cites W2014281089 @default.
- W2045335093 cites W2015289047 @default.
- W2045335093 cites W2019932147 @default.
- W2045335093 cites W2032727355 @default.
- W2045335093 cites W2037174573 @default.
- W2045335093 cites W2042196312 @default.
- W2045335093 cites W2059905343 @default.
- W2045335093 cites W2067443386 @default.
- W2045335093 cites W2087153132 @default.
- W2045335093 cites W2093517310 @default.
- W2045335093 cites W2143986155 @default.
- W2045335093 cites W2397861637 @default.
- W2045335093 doi "https://doi.org/10.1016/0006-2952(85)90560-x" @default.
- W2045335093 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2861820" @default.
- W2045335093 hasPublicationYear "1985" @default.
- W2045335093 type Work @default.
- W2045335093 sameAs 2045335093 @default.
- W2045335093 citedByCount "36" @default.
- W2045335093 crossrefType "journal-article" @default.
- W2045335093 hasAuthorship W2045335093A5004939550 @default.
- W2045335093 hasAuthorship W2045335093A5062895847 @default.
- W2045335093 hasConcept C126322002 @default.
- W2045335093 hasConcept C134018914 @default.
- W2045335093 hasConcept C159167319 @default.
- W2045335093 hasConcept C181199279 @default.
- W2045335093 hasConcept C185592680 @default.
- W2045335093 hasConcept C197957613 @default.
- W2045335093 hasConcept C25642318 @default.
- W2045335093 hasConcept C2775891158 @default.
- W2045335093 hasConcept C2909129545 @default.
- W2045335093 hasConcept C29688787 @default.
- W2045335093 hasConcept C55493867 @default.
- W2045335093 hasConcept C71924100 @default.
- W2045335093 hasConcept C86803240 @default.
- W2045335093 hasConceptScore W2045335093C126322002 @default.
- W2045335093 hasConceptScore W2045335093C134018914 @default.
- W2045335093 hasConceptScore W2045335093C159167319 @default.
- W2045335093 hasConceptScore W2045335093C181199279 @default.
- W2045335093 hasConceptScore W2045335093C185592680 @default.
- W2045335093 hasConceptScore W2045335093C197957613 @default.
- W2045335093 hasConceptScore W2045335093C25642318 @default.
- W2045335093 hasConceptScore W2045335093C2775891158 @default.
- W2045335093 hasConceptScore W2045335093C2909129545 @default.
- W2045335093 hasConceptScore W2045335093C29688787 @default.
- W2045335093 hasConceptScore W2045335093C55493867 @default.
- W2045335093 hasConceptScore W2045335093C71924100 @default.
- W2045335093 hasConceptScore W2045335093C86803240 @default.
- W2045335093 hasIssue "15" @default.
- W2045335093 hasLocation W20453350931 @default.
- W2045335093 hasLocation W20453350932 @default.
- W2045335093 hasOpenAccess W2045335093 @default.
- W2045335093 hasPrimaryLocation W20453350931 @default.
- W2045335093 hasRelatedWork W1829057012 @default.
- W2045335093 hasRelatedWork W1991194712 @default.
- W2045335093 hasRelatedWork W1992201062 @default.
- W2045335093 hasRelatedWork W2008468524 @default.
- W2045335093 hasRelatedWork W2018532868 @default.
- W2045335093 hasRelatedWork W2030608192 @default.
- W2045335093 hasRelatedWork W2041564029 @default.
- W2045335093 hasRelatedWork W2045335093 @default.
- W2045335093 hasRelatedWork W2052344298 @default.
- W2045335093 hasRelatedWork W2097047907 @default.
- W2045335093 hasVolume "34" @default.
- W2045335093 isParatext "false" @default.
- W2045335093 isRetracted "false" @default.
- W2045335093 magId "2045335093" @default.
- W2045335093 workType "article" @default.