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- W2045335905 abstract "Abstract The effect of monofluoroacetic acid (HFA) upon the respiration rate of ethanol, acetaldehyde and lactate in resting cell suspensions of yeast cells has been investigated. Comparison with similar investigations already reported with glucose and with acetate shows that whilst the respiration rate with acetaldehyde is similarly effected by monofluoroacetate as is acetate, the respiration rate with ethanol and lactate behaves more akin to the respiration rate with glucose. Thus respiration with acetaldehyde as exogenous substrate is greatly inhibited at both pH 2.2 and pH 6.8 (~ 85 per cent) whilst the respiration rate with ethanol or lactate is greatly inhibited (66 and 91 per cent respectively) at pH 2.2 but much less inhibited at pH 6.8 (29 and 64 per cent respectively). Measurements of the extent of respiration achieved by glucose and ethanol show that, despite the differences in rate of respiration at pH 2.2 and pH 6.8, the exogenous substrate is oxidized to a similar extent at both pH values. It is argued that internal citrate oxidation is inhibited to the same extent at both pH values and that the differential effects upon respiration, glucose uptake and ethanol production previously reported are consequent upon the primary block in metabolism. Measurement of citrate levels in monofluoroacetate treated cells oxidizing glucose and acetate reaffirm the conclusion and mechanisms to explain the differential effect are discussed." @default.
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- W2045335905 date "1968-11-01" @default.
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- W2045335905 title "The effect of monofluoroacetic acid upon the carbohydrate metabolism of of saccharomyces cerevisiae" @default.
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- W2045335905 doi "https://doi.org/10.1016/0006-2952(68)90042-7" @default.
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