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- W2045346749 abstract "The electrochemical response characteristics of conventional (CGE) and stearate-modified graphite paste (SGE) electrodes were examined and compared in neutral phosphate-buffered solutions containing dopamine (DA) and other potentially interfering electroactive species found in brain. Cyclic and semiderivative voltammetry at 10 mV/s (slow) and 500 mV/s (rapid) scan rates and chronoamperometry (1 s pulse duration) were used to evaluate the current-potential behavior of these electrodes before and after 20 min or 24 h insertion of the electrode surface in rat brain homogenate solutions. The extent of electrocatalytic and nucleophilic reactions of ascorbate (AA) and glutathione (GSH) on the electrochemical measurement of DA were also assessed at these electrodes. Results from the rapid-scan voltammetric and chronoamperometric experiments indicated that brain treatment of CGEs and SGEs markedly enhanced the resolving power and sensitivity to DA. Both AA and GSH significantly amplified the DA electrochemical signal at untreated SGEs but these effects were considerably attenuated following brain treatment. In addition, brain treatment of CGEs abolished the GSH-induced enhancement of the DA voltammetric signal and modified the CGE to an extent that AA catalysis of DA could be quantified at this electrode surface. These results demonstrated that the selectivity of SGEs for DA was maintained after exposure of the electrode surface to brain tissue and suggest that DA may be selectively monitored in vivo without interference from DOPAC, AA or GSH when used in combination with chronoamperometric or rapid-scan voltammetric techniques." @default.
- W2045346749 created "2016-06-24" @default.
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- W2045346749 date "1991-07-01" @default.
- W2045346749 modified "2023-10-14" @default.
- W2045346749 title "Electrochemical evaluation of stearate-modified graphite paste electrodes: selective etection of dopamine is maintained after exposure to brain tissue" @default.
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- W2045346749 doi "https://doi.org/10.1016/0022-0728(91)85270-y" @default.
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