FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045357075> ?p ?o ?g. }

• W2045357075 endingPage "911" @default.
• W2045357075 startingPage "899" @default.
• W2045357075 abstract "The rate of cereal seed starch biosynthesis is determined largely by the activity of starch biosynthetic enzymes within the endosperm. The heterotetrameric allosteric enzyme ADP-glucose pyrophosphorylase (AGP) controls the first committed step in starch synthesis, and is inhibited by phosphate (Pi) and activated by 3-PGA. An altered AGP large subunit (Sh2r6hs) from maize containing two alterations relative to the wild-type maize Sh2 sequence was introduced into wheat. The rev6 alteration confers reduced Pi inhibition and decreased heat stability in maize and the hs alteration has been shown to increase AGP subunit interactions and enzyme stability in Escherichia coli. We have previously shown that low-level expression of Sh2r6hs under control of the maize Sh2 promoter increases yield in wheat and rice. Our objective here was to examine how Sh2r6hs affects AGP activity and yield in wheat and whether further yield increases were possible with greater Sh2r6hs expression. We therefore developed an additional population of transgenic wheat expressing SH2R6HS under control of the endosperm specific wheat Glutenin (Glu) promoter. Yield increases in high-expressing Glu promoter Sh2r6hs lines were comparable to those with the Sh2 promoter despite the fact that the Glu promoter confers ∼20 times higher levels of Sh2r6hs expression than the Sh2 promoter and five times more SH2R6HS accumulation versus native wheat large AGP subunits. However, the high expression allowed endosperm AGP kinetic analysis. The I0.5 of wheat AGP was increased from 2.0 to 4.1 mM Pi by SH2R6HS expression and the A0.5 was decreased from 0.82 to 0.31 mM 3-PGA. Two properties of Sh2r6hs AGP were identified that indicate greater subunit stability: wheat AGP small subunit levels were higher without variation in small subunit transcript levels, and in vitro activity was twice as great as untransformed AGP activity after heat treatment. Both r6 and hs alterations act independently and synergistically to modify AGP allosteric-regulation, increase AGP subunit stability, and increase yield in plants expressing Sh2r6hs." @default.
• W2045357075 created "2016-06-24" @default.
• W2045357075 creator A5024415497 @default.
• W2045357075 creator A5034918187 @default.
• W2045357075 creator A5050848489 @default.
• W2045357075 creator A5073295588 @default.
• W2045357075 creator A5081816041 @default.
• W2045357075 date "2004-10-01" @default.
• W2045357075 modified "2023-10-18" @default.
• W2045357075 title "The maize Sh2r6hs ADP-glucose pyrophosphorylase (AGP) large subunit confers enhanced AGP properties in transgenic wheat (Triticum aestivum)" @default.
• W2045357075 cites W1234972026 @default.
• W2045357075 cites W1491020309 @default.
• W2045357075 cites W1552576373 @default.
• W2045357075 cites W1553868199 @default.
• W2045357075 cites W159974845 @default.
• W2045357075 cites W1796033386 @default.
• W2045357075 cites W1834235565 @default.
• W2045357075 cites W1968182221 @default.
• W2045357075 cites W1968312395 @default.
• W2045357075 cites W1971465928 @default.
• W2045357075 cites W1973562232 @default.
• W2045357075 cites W1973597156 @default.
• W2045357075 cites W1973620707 @default.
• W2045357075 cites W1980234998 @default.
• W2045357075 cites W1981266365 @default.
• W2045357075 cites W198363637 @default.
• W2045357075 cites W1990052753 @default.
• W2045357075 cites W1992907249 @default.
• W2045357075 cites W1994398462 @default.
• W2045357075 cites W2014890562 @default.
• W2045357075 cites W2017752196 @default.
• W2045357075 cites W2018288569 @default.
• W2045357075 cites W2019086791 @default.
• W2045357075 cites W2021134241 @default.
• W2045357075 cites W2025451728 @default.
• W2045357075 cites W2029285374 @default.
• W2045357075 cites W2038522857 @default.
• W2045357075 cites W2046982378 @default.
• W2045357075 cites W2047782785 @default.
• W2045357075 cites W2057120761 @default.
• W2045357075 cites W2065235676 @default.
• W2045357075 cites W2066598069 @default.
• W2045357075 cites W2080428535 @default.
• W2045357075 cites W2090710478 @default.
• W2045357075 cites W2091529610 @default.
• W2045357075 cites W2102857501 @default.
• W2045357075 cites W2122918371 @default.
• W2045357075 cites W2127459239 @default.
• W2045357075 cites W2127467738 @default.
• W2045357075 cites W2127818179 @default.
• W2045357075 cites W2135566785 @default.
• W2045357075 cites W2135579266 @default.
• W2045357075 cites W2136250987 @default.
• W2045357075 cites W2149322611 @default.
• W2045357075 cites W2163985080 @default.
• W2045357075 cites W2178751370 @default.
• W2045357075 cites W2270345981 @default.
• W2045357075 cites W2329300699 @default.
• W2045357075 cites W341661389 @default.
• W2045357075 cites W4293247451 @default.
• W2045357075 doi "https://doi.org/10.1016/j.plantsci.2004.05.031" @default.
• W2045357075 hasPublicationYear "2004" @default.
• W2045357075 type Work @default.
• W2045357075 sameAs 2045357075 @default.
• W2045357075 citedByCount "47" @default.
• W2045357075 countsByYear W20453570752012 @default.
• W2045357075 countsByYear W20453570752013 @default.
• W2045357075 countsByYear W20453570752014 @default.
• W2045357075 countsByYear W20453570752015 @default.
• W2045357075 countsByYear W20453570752016 @default.
• W2045357075 countsByYear W20453570752017 @default.
• W2045357075 countsByYear W20453570752018 @default.
• W2045357075 countsByYear W20453570752019 @default.
• W2045357075 countsByYear W20453570752020 @default.
• W2045357075 countsByYear W20453570752021 @default.
• W2045357075 countsByYear W20453570752022 @default.
• W2045357075 crossrefType "journal-article" @default.
• W2045357075 hasAuthorship W2045357075A5024415497 @default.
• W2045357075 hasAuthorship W2045357075A5034918187 @default.
• W2045357075 hasAuthorship W2045357075A5050848489 @default.
• W2045357075 hasAuthorship W2045357075A5073295588 @default.
• W2045357075 hasAuthorship W2045357075A5081816041 @default.
• W2045357075 hasConcept C102230213 @default.
• W2045357075 hasConcept C104292427 @default.
• W2045357075 hasConcept C104317684 @default.
• W2045357075 hasConcept C113442657 @default.
• W2045357075 hasConcept C122172344 @default.
• W2045357075 hasConcept C144024400 @default.
• W2045357075 hasConcept C149923435 @default.
• W2045357075 hasConcept C166342909 @default.
• W2045357075 hasConcept C177955015 @default.
• W2045357075 hasConcept C181199279 @default.
• W2045357075 hasConcept C2779687958 @default.
• W2045357075 hasConcept C28212737 @default.
• W2045357075 hasConcept C2908647359 @default.
• W2045357075 hasConcept C529335014 @default.
• W2045357075 hasConcept C55493867 @default.
• W2045357075 hasConcept C86803240 @default.

• next