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• W2045363609 abstract "Summary The significance of abscisic acid (ABA) in wound reaction and the early phase of crown gall development was studied by determining the ABA-content of treated internodes at specific intervals and by following the effect of exogenously applied ABA-solution on the growth of tumors and callus tissues. Still growing second distal internodes of Pisum sativum were used as the material. For the determination of endogenous ABA the internodes were wounded along their entire length either sterilely or with bacteria. Samples for gas-liquid chromatography were collected 0 and 20 hours as well as 2 and 4 days after the beginning of the experiment, and samples for a wheat coleoptile straight growth test after 1 and 4 days. The results indicate that wounding, both sterile and bacterial, caused changes in the inhibitor content of the tissue. Around sterile wounds the metabolism of ABA became disturbed at first. In four days' time, however, inhibitors accumulated again, and the amount of ABA may have risen above the normal level. Around inoculated wounds the catabolism of ABA was even more pronounced and the subsequent accumulation, if any, was slight. Qualitative differences in the inhibitor contents of the two kinds of tissue were also detected. It is suggested that the disturbance in the metabolism of ABA may be one of the factors releasing the DNA-synthesis in a mature tissue. The bacterial inoculation seems, amongst other things, to depress the subsequent accumulation of inhibitors. For the experiments with commercial ABA-solution one wedge-shaped wound was made per internode, and 2 μl of ABA (0.1–80 ppm) was applied both to the sterile and to the inoculated wounds either once (80 ppm) or four times (0.1, 2 and 20 ppm) at daily intervals. The size of the tumors and the amount of callus growth were determined after three weeks. None of the treatments affected the callus growth, nor did the one-dose application of inhibitor affect the tumor growth. With the four-dose treatments the response depended on the size of the plants: while in the bigger plants the tumor growth was significantly depressed, the tumors in the smaller plants thrived as well as or better than those in the untreated controls." @default.
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• W2045363609 date "1975-09-01" @default.
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• W2045363609 title "The Role of Abscisic Acid in Sterile and Crown Gall Inoculated Wounds" @default.
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• W2045363609 doi "https://doi.org/10.1016/s0044-328x(75)80022-5" @default.
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