FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045440357> ?p ?o ?g. }

• W2045440357 endingPage "9" @default.
• W2045440357 startingPage "1" @default.
• W2045440357 abstract "The in vitro proliferation of prokaryotic and eukaryotic cells is remarkably hampered in the presence of heavy water (D2O). Impairment of gene expression at the transcription or translation level can be the base for this effect. However, insights into the underlying mechanisms are lacking. Here, we employ a cell-free expression system for the quantitative analysis of the effect of increasing percentages of D2O on the kinetics of in-vitro GFP expression. Experiments are designed to discriminate the rates of transcription, translation, and protein folding using pDNA and mRNA vectors, respectively. We find that D2O significantly stimulates GFP expression at the transcription level but acts as a suppressor at translation and maturation (folding) in a linear dose-dependent manner. At a D2O concentration of 60%, the GFP expression rate was reduced to 40% of an undisturbed sample. We observed a similar inhibition of GFP expression by D2O in a recombinant Escherichia coli strain, although the inhibitory effect is less pronounced. These results demonstrate the suitability of cell-free systems for quantifying the impact of heavy water on gene expression and establish a platform to further assess the potential therapeutic use of heavy water as antiproliferative agent." @default.
• W2045440357 created "2016-06-24" @default.
• W2045440357 creator A5037604148 @default.
• W2045440357 creator A5058297742 @default.
• W2045440357 creator A5069756368 @default.
• W2045440357 creator A5089634143 @default.
• W2045440357 creator A5089996942 @default.
• W2045440357 date "2013-01-01" @default.
• W2045440357 modified "2023-10-02" @default.
• W2045440357 title "Heavy Water Reduces GFP Expression in Prokaryotic Cell-Free Assays at the Translation Level While Stimulating Its Transcription" @default.
• W2045440357 cites W1853319067 @default.
• W2045440357 cites W1966183470 @default.
• W2045440357 cites W1972391131 @default.
• W2045440357 cites W1974156076 @default.
• W2045440357 cites W1977733906 @default.
• W2045440357 cites W2006685379 @default.
• W2045440357 cites W2010000945 @default.
• W2045440357 cites W2019223981 @default.
• W2045440357 cites W2019769365 @default.
• W2045440357 cites W2026468530 @default.
• W2045440357 cites W2036742051 @default.
• W2045440357 cites W2038825677 @default.
• W2045440357 cites W2043872109 @default.
• W2045440357 cites W2044097830 @default.
• W2045440357 cites W2044165056 @default.
• W2045440357 cites W2044480314 @default.
• W2045440357 cites W2051206870 @default.
• W2045440357 cites W2057107775 @default.
• W2045440357 cites W2060480245 @default.
• W2045440357 cites W2063455767 @default.
• W2045440357 cites W2063737566 @default.
• W2045440357 cites W2074213117 @default.
• W2045440357 cites W2083480215 @default.
• W2045440357 cites W2094535327 @default.
• W2045440357 cites W2099658431 @default.
• W2045440357 cites W2123621585 @default.
• W2045440357 cites W2143146123 @default.
• W2045440357 cites W2157013651 @default.
• W2045440357 cites W2165340403 @default.
• W2045440357 cites W2325618027 @default.
• W2045440357 cites W4233808990 @default.
• W2045440357 cites W4250203876 @default.
• W2045440357 cites W1980070368 @default.
• W2045440357 doi "https://doi.org/10.1155/2013/592745" @default.
• W2045440357 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3886400" @default.
• W2045440357 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24455706" @default.
• W2045440357 hasPublicationYear "2013" @default.
• W2045440357 type Work @default.
• W2045440357 sameAs 2045440357 @default.
• W2045440357 citedByCount "16" @default.
• W2045440357 countsByYear W20454403572015 @default.
• W2045440357 countsByYear W20454403572017 @default.
• W2045440357 countsByYear W20454403572018 @default.
• W2045440357 countsByYear W20454403572019 @default.
• W2045440357 countsByYear W20454403572020 @default.
• W2045440357 countsByYear W20454403572021 @default.
• W2045440357 countsByYear W20454403572022 @default.
• W2045440357 countsByYear W20454403572023 @default.
• W2045440357 crossrefType "journal-article" @default.
• W2045440357 hasAuthorship W2045440357A5037604148 @default.
• W2045440357 hasAuthorship W2045440357A5058297742 @default.
• W2045440357 hasAuthorship W2045440357A5069756368 @default.
• W2045440357 hasAuthorship W2045440357A5089634143 @default.
• W2045440357 hasAuthorship W2045440357A5089996942 @default.
• W2045440357 hasBestOaLocation W20454403571 @default.
• W2045440357 hasConcept C104317684 @default.
• W2045440357 hasConcept C105580179 @default.
• W2045440357 hasConcept C138885662 @default.
• W2045440357 hasConcept C142613039 @default.
• W2045440357 hasConcept C149364088 @default.
• W2045440357 hasConcept C150194340 @default.
• W2045440357 hasConcept C153911025 @default.
• W2045440357 hasConcept C179926584 @default.
• W2045440357 hasConcept C185592680 @default.
• W2045440357 hasConcept C202751555 @default.
• W2045440357 hasConcept C3675279 @default.
• W2045440357 hasConcept C40767141 @default.
• W2045440357 hasConcept C41895202 @default.
• W2045440357 hasConcept C547475151 @default.
• W2045440357 hasConcept C55493867 @default.
• W2045440357 hasConcept C86803240 @default.
• W2045440357 hasConcept C95444343 @default.
• W2045440357 hasConceptScore W2045440357C104317684 @default.
• W2045440357 hasConceptScore W2045440357C105580179 @default.
• W2045440357 hasConceptScore W2045440357C138885662 @default.
• W2045440357 hasConceptScore W2045440357C142613039 @default.
• W2045440357 hasConceptScore W2045440357C149364088 @default.
• W2045440357 hasConceptScore W2045440357C150194340 @default.
• W2045440357 hasConceptScore W2045440357C153911025 @default.
• W2045440357 hasConceptScore W2045440357C179926584 @default.
• W2045440357 hasConceptScore W2045440357C185592680 @default.
• W2045440357 hasConceptScore W2045440357C202751555 @default.
• W2045440357 hasConceptScore W2045440357C3675279 @default.
• W2045440357 hasConceptScore W2045440357C40767141 @default.
• W2045440357 hasConceptScore W2045440357C41895202 @default.
• W2045440357 hasConceptScore W2045440357C547475151 @default.
• W2045440357 hasConceptScore W2045440357C55493867 @default.
• W2045440357 hasConceptScore W2045440357C86803240 @default.

• next