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- W2045449616 abstract "Abstract A method is presented for the quantitative determination of aminosugars in glycoproteins. Glycoproteins are acid hydrolyzed and reacted with the fluorogenic reagent 1-dimethylaminonaphthalene-5-sulfonyl chloride. Following cellulose thin layer electrophoresis in pyridine/acetic acid (pH 4.4), 5 h at 500 V, appropriate areas of the plate are scraped and the dansyl hexosamines are eluted with 95% ethanol. Aliquots are then applied to a silica gel tlc plate and subjected to ascending thin layer chromatography in cyclohexane/ethylacetate/ethanol (6/4/3). After spraying the plate with triethanolamine/isopropanol ( 1 4 ), fluorescent intensities are measured by in situ scanning. The aminosugar content of the glycoprotein is determined from a curve generated from a series of standards run concurrently on each plate. The method clearly resolves glucosamine from galactosamine, and is sensitive for the detection of aminosugars in the subnanomole range." @default.
- W2045449616 created "2016-06-24" @default.
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- W2045449616 date "1979-06-01" @default.
- W2045449616 modified "2023-10-10" @default.
- W2045449616 title "A fluorometric assay for the quantitation of aminosugars" @default.
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- W2045449616 doi "https://doi.org/10.1016/0003-2697(79)90768-1" @default.
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