FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045462835> ?p ?o ?g. }

• W2045462835 endingPage "89" @default.
• W2045462835 startingPage "82" @default.
• W2045462835 abstract "Immunoglobulin G (IgG) fragment crystallizable (Fc) glycosylation is crucial for antibody effector functions such as antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity. To monitor IgG Fc glycosylation, high-throughput techniques for glycosylation analysis are needed in the biotechnology industry. Here we describe the development of a fully automated high-throughput method based on glycopeptide analysis. Samples are prepared in 96-well plates. The IgG’s are purified directly from fermentation broths by means of immobilized protein A followed by trypsin digestion. Glycopeptides are purified by hydrophilic interaction solid-phase extraction and analyzed by electrospray mass spectrometry in the positive-ion mode. Data are automatically processed and relative intensities of the various IgG glycopeptides are obtained. The intermediate precision of the method is below 5% for the five major glycoforms of an IgG1 antibody. The newly developed method is suitable for glycosylation profiling of IgG’s from fermentation broths. We compared the developed method to other glycoanalytical methods and successfully applied it to analyze the fermentation time course of two different clones of the same therapeutic antibody." @default.
• W2045462835 created "2016-06-24" @default.
• W2045462835 creator A5005323074 @default.
• W2045462835 creator A5032872180 @default.
• W2045462835 creator A5039947825 @default.
• W2045462835 creator A5065397150 @default.
• W2045462835 creator A5066009283 @default.
• W2045462835 creator A5067978896 @default.
• W2045462835 creator A5089035408 @default.
• W2045462835 date "2013-01-01" @default.
• W2045462835 modified "2023-09-27" @default.
• W2045462835 title "High-throughput work flow for IgG Fc-glycosylation analysis of biotechnological samples" @default.
• W2045462835 cites W1518819083 @default.
• W2045462835 cites W1965399166 @default.
• W2045462835 cites W1968014420 @default.
• W2045462835 cites W1969824500 @default.
• W2045462835 cites W1976652440 @default.
• W2045462835 cites W1977510035 @default.
• W2045462835 cites W1980437202 @default.
• W2045462835 cites W1986197388 @default.
• W2045462835 cites W1993063472 @default.
• W2045462835 cites W1994251377 @default.
• W2045462835 cites W2001756598 @default.
• W2045462835 cites W2004303300 @default.
• W2045462835 cites W2004350398 @default.
• W2045462835 cites W2005158488 @default.
• W2045462835 cites W2011540538 @default.
• W2045462835 cites W2015723482 @default.
• W2045462835 cites W2018795884 @default.
• W2045462835 cites W2020844936 @default.
• W2045462835 cites W2023654523 @default.
• W2045462835 cites W2024166319 @default.
• W2045462835 cites W2030932286 @default.
• W2045462835 cites W2036713008 @default.
• W2045462835 cites W2050394358 @default.
• W2045462835 cites W2052491267 @default.
• W2045462835 cites W2055530646 @default.
• W2045462835 cites W2074589683 @default.
• W2045462835 cites W2075473217 @default.
• W2045462835 cites W2083344986 @default.
• W2045462835 cites W2087894213 @default.
• W2045462835 cites W2106785697 @default.
• W2045462835 cites W2124847934 @default.
• W2045462835 cites W2136007354 @default.
• W2045462835 cites W2140035322 @default.
• W2045462835 cites W2152155460 @default.
• W2045462835 cites W2155819450 @default.
• W2045462835 doi "https://doi.org/10.1016/j.ab.2012.09.032" @default.
• W2045462835 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23026777" @default.
• W2045462835 hasPublicationYear "2013" @default.
• W2045462835 type Work @default.
• W2045462835 sameAs 2045462835 @default.
• W2045462835 citedByCount "60" @default.
• W2045462835 countsByYear W20454628352012 @default.
• W2045462835 countsByYear W20454628352013 @default.
• W2045462835 countsByYear W20454628352014 @default.
• W2045462835 countsByYear W20454628352015 @default.
• W2045462835 countsByYear W20454628352016 @default.
• W2045462835 countsByYear W20454628352017 @default.
• W2045462835 countsByYear W20454628352018 @default.
• W2045462835 countsByYear W20454628352019 @default.
• W2045462835 countsByYear W20454628352020 @default.
• W2045462835 countsByYear W20454628352021 @default.
• W2045462835 countsByYear W20454628352022 @default.
• W2045462835 crossrefType "journal-article" @default.
• W2045462835 hasAuthorship W2045462835A5005323074 @default.
• W2045462835 hasAuthorship W2045462835A5032872180 @default.
• W2045462835 hasAuthorship W2045462835A5039947825 @default.
• W2045462835 hasAuthorship W2045462835A5065397150 @default.
• W2045462835 hasAuthorship W2045462835A5066009283 @default.
• W2045462835 hasAuthorship W2045462835A5067978896 @default.
• W2045462835 hasAuthorship W2045462835A5089035408 @default.
• W2045462835 hasConcept C109316439 @default.
• W2045462835 hasConcept C125823703 @default.
• W2045462835 hasConcept C137610392 @default.
• W2045462835 hasConcept C159654299 @default.
• W2045462835 hasConcept C170493617 @default.
• W2045462835 hasConcept C181199279 @default.
• W2045462835 hasConcept C185592680 @default.
• W2045462835 hasConcept C202751555 @default.
• W2045462835 hasConcept C203014093 @default.
• W2045462835 hasConcept C2777313579 @default.
• W2045462835 hasConcept C2780340462 @default.
• W2045462835 hasConcept C2780898057 @default.
• W2045462835 hasConcept C43617362 @default.
• W2045462835 hasConcept C501593827 @default.
• W2045462835 hasConcept C55493867 @default.
• W2045462835 hasConcept C86803240 @default.
• W2045462835 hasConceptScore W2045462835C109316439 @default.
• W2045462835 hasConceptScore W2045462835C125823703 @default.
• W2045462835 hasConceptScore W2045462835C137610392 @default.
• W2045462835 hasConceptScore W2045462835C159654299 @default.
• W2045462835 hasConceptScore W2045462835C170493617 @default.
• W2045462835 hasConceptScore W2045462835C181199279 @default.
• W2045462835 hasConceptScore W2045462835C185592680 @default.
• W2045462835 hasConceptScore W2045462835C202751555 @default.
• W2045462835 hasConceptScore W2045462835C203014093 @default.
• W2045462835 hasConceptScore W2045462835C2777313579 @default.

• next