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• W2045465362 abstract "We have investigated the utilization of microparticle immunoagglutination assays using forward light scattering measurements in a microfluidic chip towards detecting viral particles. The model viral target was bovine viral diarrhea virus (BVDV). Highly carboxylated polystyrene microparticles (510 nm) were coated with anti-BVDV monoclonal antibodies. This solution was in turn used to detect BVDV in diluted tissue culture media and fetal calf serum. The assay was first performed in a two well slide format for proof of concept and particle stability experiments, then in a simple y-channel microfluidic chip with optical fibers arranged in a close proximity setup. Microparticle immunoagglutination was detected via static forward light scattering measurements taken at 45° to incident light. In the microfluidic chip, BVDV was detected down to a concentration of 10 TCID50 mL−1. For assay comparison purposes reverse transcriptase polymerase chain reaction (RT-PCR) was performed on serial dilutions of the BVDV sample used in the two well and microfluidic testing. RT-PCR was effective down to a concentration of 103 TCID50 mL−1 in detecting the identical BVDV used for microfluidic assays." @default.
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• W2045465362 date "2009-05-01" @default.
• W2045465362 modified "2023-10-17" @default.
• W2045465362 title "Microfluidic immunosensor for rapid and sensitive detection of bovine viral diarrhea virus" @default.
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• W2045465362 doi "https://doi.org/10.1016/j.snb.2009.02.058" @default.
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