Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045474332> ?p ?o ?g. }
- W2045474332 abstract "Natural transformation has been described in bacterial species spread through nearly all major taxonomic groups. However, the current understanding of the structural components and the regulation of competence development is derived from only a few model organisms. Although natural transformation was discovered in members of the Actinobacteria (high GC Gram-positive bacteria) more than four decades ago, the structural components or the regulation of the competence system have not been studied in any representative of the entire phylum. In this report we identify a new role for a distinct type of pilus biogenesis genes (tad genes, for tight adherence), which so far have been connected only with biofilm formation, adherence and virulence traits. The tad-like genes found in the genome of Micrococcus luteus were shown to be required for genetic transformation in this actinobacterial species. We generated and analyzed individual knockout mutants for every open reading frame of the two predicted tad gene clusters as well as for a potential prepilin processing peptidase and identified the major component of the putative pili. By expressing a tagged variant of the major prepilin subunit and immunofluorescence microscopy we visualized filamentous structures extending from the cell surface. Our data indicate that the two tad gene islands complementarily contribute to the formation of a functional competence pilus in this organism. It seems likely that the involvement of tad genes in natural transformation is not unique only for M. luteus but may also prove to be the case in other representatives of the Actinobacteria, which contains important medically and biotechnologically relevant species." @default.
- W2045474332 created "2016-06-24" @default.
- W2045474332 creator A5020149514 @default.
- W2045474332 creator A5024597199 @default.
- W2045474332 creator A5034687804 @default.
- W2045474332 creator A5045854421 @default.
- W2045474332 creator A5051271380 @default.
- W2045474332 creator A5052571824 @default.
- W2045474332 creator A5058082024 @default.
- W2045474332 creator A5064046684 @default.
- W2045474332 creator A5086078293 @default.
- W2045474332 date "2015-02-10" @default.
- W2045474332 modified "2023-10-02" @default.
- W2045474332 title "Novel Flp pilus biogenesis-dependent natural transformation" @default.
- W2045474332 cites W1488278023 @default.
- W2045474332 cites W1542987636 @default.
- W2045474332 cites W1955724722 @default.
- W2045474332 cites W1971439989 @default.
- W2045474332 cites W1972485555 @default.
- W2045474332 cites W1976620611 @default.
- W2045474332 cites W1979471280 @default.
- W2045474332 cites W1986684101 @default.
- W2045474332 cites W1990700527 @default.
- W2045474332 cites W2004056501 @default.
- W2045474332 cites W2009645948 @default.
- W2045474332 cites W2034302623 @default.
- W2045474332 cites W2036321220 @default.
- W2045474332 cites W2039095273 @default.
- W2045474332 cites W2042894911 @default.
- W2045474332 cites W2048417870 @default.
- W2045474332 cites W2049076326 @default.
- W2045474332 cites W2056349503 @default.
- W2045474332 cites W2066352920 @default.
- W2045474332 cites W2067779211 @default.
- W2045474332 cites W2075305650 @default.
- W2045474332 cites W2080752012 @default.
- W2045474332 cites W2080884220 @default.
- W2045474332 cites W2081794308 @default.
- W2045474332 cites W2096527324 @default.
- W2045474332 cites W2104357751 @default.
- W2045474332 cites W2104878600 @default.
- W2045474332 cites W2110521895 @default.
- W2045474332 cites W2113387997 @default.
- W2045474332 cites W2114437361 @default.
- W2045474332 cites W2116475900 @default.
- W2045474332 cites W2121139898 @default.
- W2045474332 cites W2126986733 @default.
- W2045474332 cites W2131084277 @default.
- W2045474332 cites W2133467771 @default.
- W2045474332 cites W2139216324 @default.
- W2045474332 cites W2140043620 @default.
- W2045474332 cites W2145444358 @default.
- W2045474332 cites W2150011050 @default.
- W2045474332 doi "https://doi.org/10.3389/fmicb.2015.00084" @default.
- W2045474332 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4322843" @default.
- W2045474332 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25713572" @default.
- W2045474332 hasPublicationYear "2015" @default.
- W2045474332 type Work @default.
- W2045474332 sameAs 2045474332 @default.
- W2045474332 citedByCount "32" @default.
- W2045474332 countsByYear W20454743322016 @default.
- W2045474332 countsByYear W20454743322017 @default.
- W2045474332 countsByYear W20454743322018 @default.
- W2045474332 countsByYear W20454743322019 @default.
- W2045474332 countsByYear W20454743322020 @default.
- W2045474332 countsByYear W20454743322021 @default.
- W2045474332 countsByYear W20454743322022 @default.
- W2045474332 countsByYear W20454743322023 @default.
- W2045474332 crossrefType "journal-article" @default.
- W2045474332 hasAuthorship W2045474332A5020149514 @default.
- W2045474332 hasAuthorship W2045474332A5024597199 @default.
- W2045474332 hasAuthorship W2045474332A5034687804 @default.
- W2045474332 hasAuthorship W2045474332A5045854421 @default.
- W2045474332 hasAuthorship W2045474332A5051271380 @default.
- W2045474332 hasAuthorship W2045474332A5052571824 @default.
- W2045474332 hasAuthorship W2045474332A5058082024 @default.
- W2045474332 hasAuthorship W2045474332A5064046684 @default.
- W2045474332 hasAuthorship W2045474332A5086078293 @default.
- W2045474332 hasBestOaLocation W20454743321 @default.
- W2045474332 hasConcept C104317684 @default.
- W2045474332 hasConcept C131934819 @default.
- W2045474332 hasConcept C141231307 @default.
- W2045474332 hasConcept C143065580 @default.
- W2045474332 hasConcept C167625842 @default.
- W2045474332 hasConcept C174832134 @default.
- W2045474332 hasConcept C204241405 @default.
- W2045474332 hasConcept C2776683811 @default.
- W2045474332 hasConcept C2778169975 @default.
- W2045474332 hasConcept C42062724 @default.
- W2045474332 hasConcept C47289529 @default.
- W2045474332 hasConcept C523546767 @default.
- W2045474332 hasConcept C54355233 @default.
- W2045474332 hasConcept C547475151 @default.
- W2045474332 hasConcept C58123911 @default.
- W2045474332 hasConcept C60987743 @default.
- W2045474332 hasConcept C86803240 @default.
- W2045474332 hasConcept C89423630 @default.
- W2045474332 hasConcept C92938381 @default.
- W2045474332 hasConceptScore W2045474332C104317684 @default.
- W2045474332 hasConceptScore W2045474332C131934819 @default.