FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045498698> ?p ?o ?g. }

• W2045498698 endingPage "3768" @default.
• W2045498698 startingPage "3764" @default.
• W2045498698 abstract "Of the microsomal P450 cytochromes, the ethanol-inducible isoform, P450 2E1, is believed to be predominant in leading to oxidative damage, including the generation of radical species that contribute to lipid peroxidation, and in the reductive beta-scission of lipid hydroperoxides to give hydrocarbons and aldehydes. In the present study, the sensitivity of a series of P450s to trans-4-hydroxy-2-nonenal (HNE), a known toxic product of membrane lipid peroxidation, was determined. After incubation of a purified cytochrome with HNE, the other components of the reconstituted system (NADPH-cytochrome P450 reductase, phosphatidylcholine, and NADPH) were added, and the rate of oxygenation of 1-phenylethanol to yield acetophenone was assayed. Inactivation occurs in a time-dependent and HNE concentration-dependent manner, with P450s 2E1 and 1A1 being the most sensitive, followed by isoforms 1A2, 3A6, and 2B4. At an HNE concentration of 0.24 microM, which was close to the micromolar concentration of the enzyme, four of the isoforms were significantly inhibited, but not P450 2B4. In other experiments, the reductase was shown to be only relatively weakly inactivated by HNE. P450s 2E1 and 2B4 in microsomal membranes from animals induced with acetone or phenobarbital, respectively, are as readily inhibited as the purified forms. Evidence was obtained that the P450 heme is apparently not altered and the sulfur ligand is not displaced, that substrate protects against HNE, and that the inactivation is reversed upon dialysis. Higher levels of reductase or substrate do not restore the activity of inhibited P450 in the catalytic assay. Our results suggest that the observed inhibition of the various P450s is of sufficient magnitude to cause significant changes in the metabolism of foreign compounds such as drugs and chemical carcinogens by the P450 oxygenase system at HNE concentrations that occur in biological membranes. In view of the known activities of P450 2E1 in generating lipid hydroperoxides and in their beta-scission, its inhibition by this product of membrane peroxidation may provide a negative regulatory function." @default.
• W2045498698 created "2016-06-24" @default.
• W2045498698 creator A5009848406 @default.
• W2045498698 creator A5011112140 @default.
• W2045498698 creator A5059725315 @default.
• W2045498698 date "1995-04-25" @default.
• W2045498698 modified "2023-10-16" @default.
• W2045498698 title "Inactivation of ethanol-inducible cytochrome P450 and other microsomal P450 isozymes by trans-4-hydroxy-2-nonenal, a major product of membrane lipid peroxidation." @default.
• W2045498698 cites W1012908378 @default.
• W2045498698 cites W1502736133 @default.
• W2045498698 cites W1508566072 @default.
• W2045498698 cites W1522000230 @default.
• W2045498698 cites W1533807800 @default.
• W2045498698 cites W1547083521 @default.
• W2045498698 cites W1650427240 @default.
• W2045498698 cites W1839868945 @default.
• W2045498698 cites W1978135825 @default.
• W2045498698 cites W1978896699 @default.
• W2045498698 cites W1987345891 @default.
• W2045498698 cites W1994914880 @default.
• W2045498698 cites W1998287241 @default.
• W2045498698 cites W1998645487 @default.
• W2045498698 cites W2002630124 @default.
• W2045498698 cites W2014208807 @default.
• W2045498698 cites W2016864577 @default.
• W2045498698 cites W2028181410 @default.
• W2045498698 cites W2042876924 @default.
• W2045498698 cites W2054498117 @default.
• W2045498698 cites W2057522312 @default.
• W2045498698 cites W2057685857 @default.
• W2045498698 cites W2062364743 @default.
• W2045498698 cites W2075303826 @default.
• W2045498698 cites W2093277237 @default.
• W2045498698 cites W2150946709 @default.
• W2045498698 cites W2414208602 @default.
• W2045498698 cites W2462123524 @default.
• W2045498698 cites W324750310 @default.
• W2045498698 cites W76102735 @default.
• W2045498698 doi "https://doi.org/10.1073/pnas.92.9.3764" @default.
• W2045498698 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/42042" @default.
• W2045498698 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7731980" @default.
• W2045498698 hasPublicationYear "1995" @default.
• W2045498698 type Work @default.
• W2045498698 sameAs 2045498698 @default.
• W2045498698 citedByCount "35" @default.
• W2045498698 countsByYear W20454986982014 @default.
• W2045498698 countsByYear W20454986982015 @default.
• W2045498698 countsByYear W20454986982017 @default.
• W2045498698 countsByYear W20454986982023 @default.
• W2045498698 crossrefType "journal-article" @default.
• W2045498698 hasAuthorship W2045498698A5009848406 @default.
• W2045498698 hasAuthorship W2045498698A5011112140 @default.
• W2045498698 hasAuthorship W2045498698A5059725315 @default.
• W2045498698 hasBestOaLocation W20454986981 @default.
• W2045498698 hasConcept C119795356 @default.
• W2045498698 hasConcept C134651460 @default.
• W2045498698 hasConcept C181199279 @default.
• W2045498698 hasConcept C185592680 @default.
• W2045498698 hasConcept C18903297 @default.
• W2045498698 hasConcept C2776217839 @default.
• W2045498698 hasConcept C2777289219 @default.
• W2045498698 hasConcept C2780829032 @default.
• W2045498698 hasConcept C526171541 @default.
• W2045498698 hasConcept C55493867 @default.
• W2045498698 hasConcept C86803240 @default.
• W2045498698 hasConcept C87644729 @default.
• W2045498698 hasConceptScore W2045498698C119795356 @default.
• W2045498698 hasConceptScore W2045498698C134651460 @default.
• W2045498698 hasConceptScore W2045498698C181199279 @default.
• W2045498698 hasConceptScore W2045498698C185592680 @default.
• W2045498698 hasConceptScore W2045498698C18903297 @default.
• W2045498698 hasConceptScore W2045498698C2776217839 @default.
• W2045498698 hasConceptScore W2045498698C2777289219 @default.
• W2045498698 hasConceptScore W2045498698C2780829032 @default.
• W2045498698 hasConceptScore W2045498698C526171541 @default.
• W2045498698 hasConceptScore W2045498698C55493867 @default.
• W2045498698 hasConceptScore W2045498698C86803240 @default.
• W2045498698 hasConceptScore W2045498698C87644729 @default.
• W2045498698 hasIssue "9" @default.
• W2045498698 hasLocation W20454986981 @default.
• W2045498698 hasLocation W20454986982 @default.
• W2045498698 hasLocation W20454986983 @default.
• W2045498698 hasLocation W20454986984 @default.
• W2045498698 hasOpenAccess W2045498698 @default.
• W2045498698 hasPrimaryLocation W20454986981 @default.
• W2045498698 hasRelatedWork W1963853438 @default.
• W2045498698 hasRelatedWork W1970867325 @default.
• W2045498698 hasRelatedWork W1974172069 @default.
• W2045498698 hasRelatedWork W2007137161 @default.
• W2045498698 hasRelatedWork W2024889190 @default.
• W2045498698 hasRelatedWork W2030028187 @default.
• W2045498698 hasRelatedWork W2034361214 @default.
• W2045498698 hasRelatedWork W2036206840 @default.
• W2045498698 hasRelatedWork W2051212618 @default.
• W2045498698 hasRelatedWork W281468747 @default.
• W2045498698 hasVolume "92" @default.
• W2045498698 isParatext "false" @default.
• W2045498698 isRetracted "false" @default.

• next