Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045504992> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2045504992 endingPage "530" @default.
- W2045504992 startingPage "517" @default.
- W2045504992 abstract "The fibrous sheath was isolated from epididymal spermatozoa of rat caput epididymidis and cauda epididymidis and its polypeptide composition and subunit substructure determined. Cauda epididymal spermatozoa were extracted sequentially with Triton X-100-dithiothreitol (DDT), potassium thiocyanate-DTT, and urea-DTT; the loss of specific flagellar components as determined by electron microscopy was correlated with the solubilization of specific polypeptide components as determined by SDS-polyacrylamide gel electrophoresis. Triton X-100 treatment solubilized all flagellar membranes but had no effect on the flagellar structural elements. KSCN extraction affected the organization of the 9+2 axoneme, solubilizing the central pair of microtubules, portions of the b-subtubule and disorganizing the remaining axoneme matrix components. KSCN also disorganized the outer dense fiber cortex but did not remove it. SDS gels show that none of the major polypeptide bands present in Triton extracted sperm were removed by KSCN extraction. Brief urea extraction resulted in solubilization of specific outer dense fiber polypeptides; the nonsolubilized portion of the outer dense fiber assumed a very regular corkscrew configuration. Prolonged urea extraction completely solubilized the outer dense fibers and connecting piece, leaving the fibrous sheath as the only intact flagellar organelle. Caput epididymal spermatozoa were treated sequentially with Triton X-100 and sodium dodecyl sulfate (SDS), and the fibrous sheath was recovered by differential centrifugation. SDS-polyacryladmide gel electrophoresis showed that the fibrous sheath of rat sperm is composed predominantly of a single polypeptide with a molecular weight of 80,000 daltons. Study of the substructure of the fibrous sheath, by negative staining of demembranated spermatozoa, show that the ribs are composed of parallel 5–6 nm wide filaments which appear to have a beaded substructure." @default.
- W2045504992 created "2016-06-24" @default.
- W2045504992 creator A5011889083 @default.
- W2045504992 creator A5029643203 @default.
- W2045504992 creator A5076718225 @default.
- W2045504992 date "1976-06-01" @default.
- W2045504992 modified "2023-10-06" @default.
- W2045504992 title "Isolation and Characterization of the Fibrous Sheath of Rat Epididymal Spermatozoa1" @default.
- W2045504992 doi "https://doi.org/10.1095/biolreprod14.5.517" @default.
- W2045504992 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1276316" @default.
- W2045504992 hasPublicationYear "1976" @default.
- W2045504992 type Work @default.
- W2045504992 sameAs 2045504992 @default.
- W2045504992 citedByCount "80" @default.
- W2045504992 countsByYear W20455049922013 @default.
- W2045504992 countsByYear W20455049922014 @default.
- W2045504992 countsByYear W20455049922016 @default.
- W2045504992 countsByYear W20455049922017 @default.
- W2045504992 countsByYear W20455049922019 @default.
- W2045504992 crossrefType "journal-article" @default.
- W2045504992 hasAuthorship W2045504992A5011889083 @default.
- W2045504992 hasAuthorship W2045504992A5029643203 @default.
- W2045504992 hasAuthorship W2045504992A5076718225 @default.
- W2045504992 hasBestOaLocation W20455049921 @default.
- W2045504992 hasConcept C102362939 @default.
- W2045504992 hasConcept C104292427 @default.
- W2045504992 hasConcept C104317684 @default.
- W2045504992 hasConcept C105702510 @default.
- W2045504992 hasConcept C115811362 @default.
- W2045504992 hasConcept C116084860 @default.
- W2045504992 hasConcept C12554922 @default.
- W2045504992 hasConcept C132946820 @default.
- W2045504992 hasConcept C168240541 @default.
- W2045504992 hasConcept C181199279 @default.
- W2045504992 hasConcept C185592680 @default.
- W2045504992 hasConcept C24870808 @default.
- W2045504992 hasConcept C2777824588 @default.
- W2045504992 hasConcept C2778913249 @default.
- W2045504992 hasConcept C41625074 @default.
- W2045504992 hasConcept C43617362 @default.
- W2045504992 hasConcept C4725764 @default.
- W2045504992 hasConcept C54355233 @default.
- W2045504992 hasConcept C55493867 @default.
- W2045504992 hasConcept C74864618 @default.
- W2045504992 hasConcept C7623868 @default.
- W2045504992 hasConcept C86803240 @default.
- W2045504992 hasConcept C87555872 @default.
- W2045504992 hasConceptScore W2045504992C102362939 @default.
- W2045504992 hasConceptScore W2045504992C104292427 @default.
- W2045504992 hasConceptScore W2045504992C104317684 @default.
- W2045504992 hasConceptScore W2045504992C105702510 @default.
- W2045504992 hasConceptScore W2045504992C115811362 @default.
- W2045504992 hasConceptScore W2045504992C116084860 @default.
- W2045504992 hasConceptScore W2045504992C12554922 @default.
- W2045504992 hasConceptScore W2045504992C132946820 @default.
- W2045504992 hasConceptScore W2045504992C168240541 @default.
- W2045504992 hasConceptScore W2045504992C181199279 @default.
- W2045504992 hasConceptScore W2045504992C185592680 @default.
- W2045504992 hasConceptScore W2045504992C24870808 @default.
- W2045504992 hasConceptScore W2045504992C2777824588 @default.
- W2045504992 hasConceptScore W2045504992C2778913249 @default.
- W2045504992 hasConceptScore W2045504992C41625074 @default.
- W2045504992 hasConceptScore W2045504992C43617362 @default.
- W2045504992 hasConceptScore W2045504992C4725764 @default.
- W2045504992 hasConceptScore W2045504992C54355233 @default.
- W2045504992 hasConceptScore W2045504992C55493867 @default.
- W2045504992 hasConceptScore W2045504992C74864618 @default.
- W2045504992 hasConceptScore W2045504992C7623868 @default.
- W2045504992 hasConceptScore W2045504992C86803240 @default.
- W2045504992 hasConceptScore W2045504992C87555872 @default.
- W2045504992 hasIssue "5" @default.
- W2045504992 hasLocation W20455049921 @default.
- W2045504992 hasLocation W20455049922 @default.
- W2045504992 hasOpenAccess W2045504992 @default.
- W2045504992 hasPrimaryLocation W20455049921 @default.
- W2045504992 hasRelatedWork W146430892 @default.
- W2045504992 hasRelatedWork W1502019371 @default.
- W2045504992 hasRelatedWork W1989061429 @default.
- W2045504992 hasRelatedWork W1999619046 @default.
- W2045504992 hasRelatedWork W2016063783 @default.
- W2045504992 hasRelatedWork W2029981677 @default.
- W2045504992 hasRelatedWork W2064573683 @default.
- W2045504992 hasRelatedWork W2077094385 @default.
- W2045504992 hasRelatedWork W2117343967 @default.
- W2045504992 hasRelatedWork W2808253106 @default.
- W2045504992 hasVolume "14" @default.
- W2045504992 isParatext "false" @default.
- W2045504992 isRetracted "false" @default.
- W2045504992 magId "2045504992" @default.
- W2045504992 workType "article" @default.