Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045507265> ?p ?o ?g. }
- W2045507265 abstract "Toxoplasmosis is caused by the apicomplexan parasite Toxoplasma gondii and can be acquired either congenitally or via the oral route. In the latter case, transmission is mediated by two distinct invasive stages, i.e., bradyzoites residing in tissue cysts or sporozoites contained in environmentally resistant oocysts shed by felids in their feces. The oocyst plays a central epidemiological role, yet this stage has been scarcely investigated at the molecular level and the knowledge of its expressed proteome is very limited.Using one-dimensional gel electrophoresis coupled to liquid chromatography-linked tandem mass spectrometry, we analysed total or fractionated protein extracts of partially sporulated T. gondii oocysts, producing a dataset of 1304 non reduntant proteins (~18% of the total predicted proteome), ~59% of which were classified according to the MIPS functional catalogue database. Notably, the comparison of the oocyst dataset with the extensively covered proteome of T. gondii tachyzoite, the invasive stage responsible for the clinical signs of toxoplasmosis, identified 154 putative oocyst/sporozoite-specific proteins, some of which were validated by Western blot. The analysis of this protein subset showed that, compared to tachyzoites, oocysts have a greater capability of de novo amino acid biosynthesis and are well equipped to fuel the Krebs cycle with the acetyl-CoA generated through fatty acid β-oxidation and the degradation of branched amino acids.The study reported herein significantly expanded our knowledge of the proteome expressed by the oocyst/sporozoite of T. gondii, shedding light on a stage-specifc subset of proteins whose functional profile is consistent with the adaptation of T. gondii oocysts to the nutrient-poor and stressing extracellular environment." @default.
- W2045507265 created "2016-06-24" @default.
- W2045507265 creator A5033329876 @default.
- W2045507265 creator A5038538768 @default.
- W2045507265 creator A5042093756 @default.
- W2045507265 creator A5058778173 @default.
- W2045507265 creator A5061845168 @default.
- W2045507265 creator A5062271097 @default.
- W2045507265 creator A5070787894 @default.
- W2045507265 creator A5077379629 @default.
- W2045507265 date "2013-03-15" @default.
- W2045507265 modified "2023-10-16" @default.
- W2045507265 title "Global proteomic analysis of the oocyst/sporozoite of Toxoplasma gondiireveals commitment to a host-independent lifestyle" @default.
- W2045507265 cites W1605944146 @default.
- W2045507265 cites W1760381734 @default.
- W2045507265 cites W1884087341 @default.
- W2045507265 cites W1916169393 @default.
- W2045507265 cites W1964706690 @default.
- W2045507265 cites W1967567650 @default.
- W2045507265 cites W1981610800 @default.
- W2045507265 cites W1989316936 @default.
- W2045507265 cites W1996336718 @default.
- W2045507265 cites W1996338896 @default.
- W2045507265 cites W1998347692 @default.
- W2045507265 cites W2001150659 @default.
- W2045507265 cites W2011399080 @default.
- W2045507265 cites W2011603667 @default.
- W2045507265 cites W2014463389 @default.
- W2045507265 cites W2025345440 @default.
- W2045507265 cites W2026051186 @default.
- W2045507265 cites W2027308346 @default.
- W2045507265 cites W2027844333 @default.
- W2045507265 cites W2033846060 @default.
- W2045507265 cites W2034341346 @default.
- W2045507265 cites W2041028276 @default.
- W2045507265 cites W2041649340 @default.
- W2045507265 cites W2047308369 @default.
- W2045507265 cites W2050263490 @default.
- W2045507265 cites W2059312531 @default.
- W2045507265 cites W2061662768 @default.
- W2045507265 cites W2062809344 @default.
- W2045507265 cites W2067197374 @default.
- W2045507265 cites W2067478890 @default.
- W2045507265 cites W2070560022 @default.
- W2045507265 cites W2073645035 @default.
- W2045507265 cites W2079084833 @default.
- W2045507265 cites W2079843249 @default.
- W2045507265 cites W2080018424 @default.
- W2045507265 cites W2080824721 @default.
- W2045507265 cites W2082073529 @default.
- W2045507265 cites W2082460216 @default.
- W2045507265 cites W2083692164 @default.
- W2045507265 cites W2085827012 @default.
- W2045507265 cites W2090036244 @default.
- W2045507265 cites W2092661540 @default.
- W2045507265 cites W2092943158 @default.
- W2045507265 cites W2110145073 @default.
- W2045507265 cites W2110935155 @default.
- W2045507265 cites W2114241062 @default.
- W2045507265 cites W2114671362 @default.
- W2045507265 cites W2118013877 @default.
- W2045507265 cites W2118103211 @default.
- W2045507265 cites W2126000073 @default.
- W2045507265 cites W2134864542 @default.
- W2045507265 cites W2135382295 @default.
- W2045507265 cites W2142713800 @default.
- W2045507265 cites W2142788740 @default.
- W2045507265 cites W2153072978 @default.
- W2045507265 cites W2153756387 @default.
- W2045507265 cites W2161177862 @default.
- W2045507265 cites W2171269243 @default.
- W2045507265 cites W2171756247 @default.
- W2045507265 cites W2172006456 @default.
- W2045507265 cites W2619378031 @default.
- W2045507265 doi "https://doi.org/10.1186/1471-2164-14-183" @default.
- W2045507265 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3616887" @default.
- W2045507265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23496850" @default.
- W2045507265 hasPublicationYear "2013" @default.
- W2045507265 type Work @default.
- W2045507265 sameAs 2045507265 @default.
- W2045507265 citedByCount "50" @default.
- W2045507265 countsByYear W20455072652014 @default.
- W2045507265 countsByYear W20455072652015 @default.
- W2045507265 countsByYear W20455072652016 @default.
- W2045507265 countsByYear W20455072652017 @default.
- W2045507265 countsByYear W20455072652018 @default.
- W2045507265 countsByYear W20455072652019 @default.
- W2045507265 countsByYear W20455072652020 @default.
- W2045507265 countsByYear W20455072652021 @default.
- W2045507265 countsByYear W20455072652022 @default.
- W2045507265 crossrefType "journal-article" @default.
- W2045507265 hasAuthorship W2045507265A5033329876 @default.
- W2045507265 hasAuthorship W2045507265A5038538768 @default.
- W2045507265 hasAuthorship W2045507265A5042093756 @default.
- W2045507265 hasAuthorship W2045507265A5058778173 @default.
- W2045507265 hasAuthorship W2045507265A5061845168 @default.
- W2045507265 hasAuthorship W2045507265A5062271097 @default.
- W2045507265 hasAuthorship W2045507265A5070787894 @default.
- W2045507265 hasAuthorship W2045507265A5077379629 @default.
- W2045507265 hasBestOaLocation W20455072651 @default.